FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC179CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC179CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30818
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGACTGTCCAGCAGCAAAGTTGGTGTTGCTGATGTTAACATATGTC540.17522227269777402No Hit
CAGCAAAGTTGGTGTTGCTGATGTTAACATATGTCTGATTTACCCAAGTG330.10708027775975079No Hit
GTGTTGTTTTCATAAGTAATGACGCTTTGATTGCATGTTTCAATCTGATT330.10708027775975079No Hit
GTTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTG330.10708027775975079No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC320.10383542085794016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT500.095.000011
CGTAGAG254.5140405E-795.000011
TCTCGAC500.095.000018
CTACGAC254.5140405E-795.000016
CGACTGC254.5140405E-795.000019
GTAGATC156.556411E-495.02
GTATGTA550.095.02
GTGTCGT550.095.02
GTCGTCT450.095.04
GTACTCA450.095.09
TCGCGCG156.556411E-495.03
GCGCGTC156.556411E-495.05
CAGAGTA156.556411E-495.08
GACATAC353.1650416E-1095.02
ACTCACG156.556411E-495.07
GAGTATA301.19234755E-895.02
ACATACT156.556411E-495.03
CGTATGT550.095.01
GCGTCAC156.556411E-495.07
CGTGTCG600.095.01
Produced by FastQC (version 0.11.5)