FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC160CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC160CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59363
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATACTC1270.2139379748328083No Hit
CGCTGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAAT1260.21225342384987284No Hit
CGCTGACGTACACTCTAGTATCCTGACCACTCATTTATTCCTACGATATC1210.20383066893519533No Hit
CGCTGACGTACACTCTAGTATCCTACGATATCTTGCTTTATTGAGAAGTT900.15160958846419487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGA850.095.000017
CGTCTCG850.095.000016
CTCACGT156.586774E-495.03
CTCACGC301.2118107E-895.08
CGAGTAG550.095.01
TAGACGT500.095.09
GAGTACG156.586774E-495.06
CGTTCCC156.586774E-495.07
GCCGACG156.586774E-495.02
TGACATA156.586774E-495.04
CGCTACA156.586774E-495.01
ACTACGA500.095.05
GTACTCA301.2118107E-895.09
ACGCTGT254.5665183E-795.07
TCGTACT301.2118107E-895.07
CGTGTCG1100.095.01
CTACACG156.586774E-495.03
GCTACAC156.586774E-495.02
ATAGTCG353.237801E-1095.03
ATACGTG450.095.09
Produced by FastQC (version 0.11.5)