FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC154CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC154CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278541
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCCCTCTATTATCTCAAATCTGTGCTTTAAAAGTGCATTTGGGTCCTC13860.4975928139842967No Hit
CGTCACCTCTAACTGCTTTTACCATGCAATCTTCTTGTGAGAATACCATT8640.31018772819800317No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAGAGAACGTACGTTTCGACC7900.2836207236995631No Hit
CGTCACGTCATGTCAGTTAGGTACCGCGAAGCAAGTGCGGAGGCCATGGT6690.24018008120887052No Hit
CGTCACGTCGTGTACGTTCTGGAAAGGAAGACTGCTGTTTATAGCTCCCT4450.15976104056494375No Hit
CGTCACGTCGTGTTCGGTGACAATCCGCGTCCCGAAGATGGAGAGGGCAG3500.12565475100613555No Hit
CGTCACGTCGTGGAAGCAAGTATTGGAAGAGCTGCAGGACATTGAGAGTG3330.1195515202429804No Hit
CGTCACGTCGTGTTTGGATAGGAAGGACCAAAAGTAACAGACTTAGAAAG2980.10698604514236684No Hit
CGTCACGTCGTGAGCGTGAACACAAATCCTAAAATCCCCTTAGTCAGAGG2800.10052380080490843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC254.611411E-795.000014
ATCTATA254.611411E-795.000017
TATCTAT254.611411E-795.000016
GTATCTA254.611411E-795.000015
CGCACGT254.611411E-795.000011
AGCTACG254.611411E-795.000015
CTCACGC550.095.08
GTCGATA550.095.02
CGTCTTG156.6126167E-495.06
CCGTCGT156.6126167E-495.05
ACGATAC156.6126167E-495.05
CCCGTCG156.6126167E-495.04
GAGACGT4600.095.02
CGCTATC1150.095.01
CGCTATA156.6126167E-495.01
CGCTACA700.095.01
ACGTCTT353.2923708E-1095.05
ACGTCAT1850.095.05
CGTCGAT550.095.01
CGTCCTA156.6126167E-495.06
Produced by FastQC (version 0.11.5)