FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC152CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC152CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50753
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAGAGATATAGACGTAGACAGAGCAGTCAAATGTTATCTCCCTCTT4260.8393592496995252No Hit
CGCGTAGAGATATAGACGTAGAATGAGAAAATGTGAATGTGTGTCTTCTC2550.5024333536933777No Hit
CGCGTAGAGATATAGACGTAGATTTTTGGATAGTGAATAGTACTTGCCAC1180.23249857151301398No Hit
CGCGTAGAGATATAGACTTAGAAAGGGGTTTGAGATGATTTGGGATCCTA620.1221602663881938No Hit
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT520.10245699761590447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAC254.556896E-795.000013
GCTATCA254.556896E-795.000012
CGCGCGT409.094947E-1295.000014
CGCGCAC800.095.000016
GCGCACT800.095.000017
ATCGTCT201.7277443E-595.000016
ATACTCT409.094947E-1295.000018
CGTAGTA254.556896E-795.000011
CGCTATC254.556896E-795.000011
CACTACT254.556896E-795.000017
CATACGA201.7277443E-595.000018
CGTGTAT409.094947E-1295.000011
ATACGAC409.094947E-1295.000019
TATACTC409.094947E-1295.000017
TCGCGCG409.094947E-1295.000013
GTAGTAG254.556896E-795.000012
CGTATAG600.095.01
GCGCGTC353.2196112E-1095.05
GTCTACT156.5812183E-495.06
ACGATGT301.2081728E-895.05
Produced by FastQC (version 0.11.5)