FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC149CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC149CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625116
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATAGACGACTAGTACGTGAATGAGAAAATGTGAATGTGTGTCTTCTC61750.9878166612276761No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGGTGTTTTTTATCA23850.3815291881826733No Hit
CGTATAGACGACTAGTACGTGAAGGACCAAAAGTAACAGACTTAGAAAGG20520.32825907511565855No Hit
CGTATAGACGACTAGTACGTGAATGAGCAAATGTGAATGTGTGTCTTCTC16020.25627243583590886No Hit
CGTATAGACGACTAGTACGTGACCCAGAAGCAAGGTCTTATACAGTCCAA15530.24843389066989163No Hit
CGTATAGACGACTAGTACGTGACGCCACAAAAAGAAATGCTGCTCCCACT14530.2324368597188362No Hit
CGTATAGACGACTAGTACGTGAATGTGTGTCTTCTCAGATTTAATTTTGT11960.1913244901746236No Hit
CGTATAGACGACTAGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT11850.1895648167700075No Hit
CGTATAGACGACTAGTACGTGATTCACCCAGGTGCTGTTTTCATATGTGA11720.18748520274637026No Hit
CGTATAGACGACTAGTACGTGAACACAAATCCTAAAATCCCCTTAGTCAG11260.18012656850888475No Hit
CGTATAGACGACTAGTACGTGAGGGGCGGGAACAACTAGGTCAGAAGTTT10630.1700484390097198No Hit
CGTATAGACGACAGAGAATAGTTTTGAGCAAATAACATTTATGCAAGCAT10580.16924858746216703No Hit
CGTATAGACGACTAGTACGTGACAGAGCAGTCAAATGTTATCTCCCTCTT10200.16316971570076594No Hit
CGTATAGACGACTAGTACGTGAAAGAAATTTCAGTGTCCTGGGAGTTGGT10040.16061019074859706No Hit
CGTATAGACGACTAGTACGTGACCGAGAGTGCTGCCTCTTCCCTTTAGAG8930.14285348639292547No Hit
CGTATAGACGACTAGTACGTGAAACAGGATGTTGGACGAAACTTCCGCTG8480.1356548224649505No Hit
CGTATAGACGACTAGTACGTGACTTAGAAAGGGGTTTGAGATGATTTGGG8260.13213547565571832No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGTAGTTTTTTACTC7880.12605660389431722No Hit
CGTATAGACGACTAGTACGTGACTTCCAGTTTGGATTGAGTGACTAGCCC7730.1236570492516589No Hit
CGTATAGACGACTAGTACGTGAAAACCCTTTTACTCCGTTTGCTCCATCA7630.12205734615655334No Hit
CGTATAGACGACTAGTACGTGAAAGGGGTTTGAGATGATTTGGGATCCTA7310.11693829625221558No Hit
CGTATAGACGACTAGTACGTGAACGCCACAAAAAGAAATGCTGCTCCCAC7300.11677832594270503No Hit
CGTATAGACGACTAGTACGTGAGGGGCCGCAAGTATAATTGGGATTGTGC6540.10462058241990287No Hit
CGTATAGACGACTAGTACGTGTGATCAGTAGAAACAAGGGTGTTTTTTAT6460.10334081994381843No Hit
CGTATAGACGACTAGTACGTGAAGGGCGGGAACAACTAGGTCAGAAGTTT6380.10206105746773399No Hit
CGTATAGACGACTAGTACGTGACGCGTGATCAGTAGAAACAAGGGTGTTT6280.10046135437262844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG750.095.09
ACGATTA750.095.08
CTACGTC301.231092E-895.07
CTACGAC301.231092E-895.06
CATACGA301.231092E-895.08
TATAGCG254.6181776E-795.03
CGTATTG409.094947E-1294.999991
CACGTCG4600.093.9673844
GTGTCGC4350.093.908042
CGTATAG318550.093.449231
CGTGTCG5200.092.259621
CGTGTGC4450.091.797759
TGTCGCG4450.091.797753
GCGCGTC3600.091.041675
CGCGCAC4400.090.681826
GTATAGA328750.090.434222
CGTGTAT2950.090.1694951
TCACGTC4850.090.1030963
CGCGCGT3650.089.794524
TAGCCGA850.089.411765
Produced by FastQC (version 0.11.5)