FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC140CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC140CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238833
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCCCTCTATTATCTCAAATCTGTGCTTTAAAAGTGCATTTGGGTCCTC35351.4801137196283596No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAGAGAACGTACGTTTCGACC23140.968877835140035No Hit
GGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTG7010.29351052827708063No Hit
CGTCGCGCCTCTTTCGGACTGACGAAAGGAGTCCCAAAGACCTCTGCTTG4640.19427801015772528No Hit
CGTCGCGCGCCAAGGTATGCTTTCGCACTGAGTAGAGGCTTTGGATCAGG4540.1900909840767398No Hit
CGTCCCTCTATTATCTCAAATCTGTGCTTTAAAAGTGCATTTGGATCCTC4350.18213563452286746No Hit
CGTCGCGCGTCCCGACTCCTTTGACTGCGGCGCCTGCTGCTCCAGATCTT3630.15198904673977215No Hit
GACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA3500.14654591283449106No Hit
CGTCCCTCTATTATCTCAAATCTGTGCTTTAAAAGTGCATTTGGCTCCTC3280.13733445545632303No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA3190.13356613198343611No Hit
TGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGT2670.11179359636231173No Hit
AGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC2640.11053748853801611No Hit
CGTCGCGCGTCCCGAAGATGGAGAGGGCAGCTGCAATCCAGTGACTGTTG2500.10467565202463647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA201.7409891E-595.000019
GTGACGT201.7409891E-595.000014
CAGAGTA409.094947E-1295.000018
GTACGCT201.7409891E-595.000015
ATCTATA156.611452E-495.07
CGAGTAG500.095.01
GCGCGTA254.609392E-795.05
ACGATGT1300.095.05
CGCTATC1050.095.01
CGCTATA353.2923708E-1095.01
GTATCTA156.611452E-495.05
CGTGTCG3400.095.01
TAGATAC1150.095.06
CTACACG450.095.03
TACACGC450.095.04
TCGCGCT156.611452E-495.03
TCGCGCC550.095.03
TCACGTC4500.095.03
CACGTCG4450.093.932584
CGTGTGC4200.093.869059
Produced by FastQC (version 0.11.5)