FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC139CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC139CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149773
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGTAGATACGACAGAGAGACAGAGCAGTCAAATGTTATCTCCCTCTT15011.002183304066821No Hit
CGTAGTAGATACGACAGAGAGAATGAGAAAATGTGAATGTGTGTCTTCTC6030.4026092820468309No Hit
CGTAGTAGATACGACAGAGAGAAGTCAAACTTTATCTCCCTCTTAAGCTT4430.2957809485020665No Hit
CGTAGTAGATACGACAGAGAGATGAATTGCCAGCCAATGTCACTGAAGTT3510.23435465671382694No Hit
CGTAGTAGATACGACAGAGAGACGCCACAAAAAGAAATGCTGCTCCCACT2820.18828493787264727No Hit
CGTAGTAGATACGACAGAGAGAGGGGTTTGAGATGATTTGGGATCCTAAT2600.17359604201024217No Hit
CGTAGTAGATACGACAGAGAGACTGCTCTGTCCATATTATTTGGATCCCC2450.1635808857404205No Hit
CGTAGTAGATACGACAGAGAGAATGCCAGATCATAATGTGAGTCAAACCA2340.15623643780921795No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCCTGACGGGATGATAGAGAGAAC2240.14955966696267017No Hit
CGTAGTAGATACGACAGAGAGAAGTAGAAACAAGGTAGTTTTTTACTCTA1850.12352026066113385No Hit
CGTAGTAGATACGACAGAGAGAGGATTCTTCTTTCAGAATCCTCTCTACT1750.11684348981458607No Hit
CGTAGTAGATACGACAGAGAGAAGGGGTTTGAGATGATTTGGGATCCTAA1730.11550813564527651No Hit
CGTAGTAGATACGACAGAGAGATTTTTGGATAGTGAATAGTACTTGCCAC1570.10482530229080009No Hit
CGTAGTAGATACGACAGTGATTTCTCAGTGAAACAGGATGTTGTAGCAAT1560.10415762520614531No Hit
CGTAGTAGATACGACCGAGAGTGCTGCCTCTTCCCTTTAGAGACTTCTGA1550.10348994812149054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA254.6009336E-795.000011
AGATACA900.095.000017
GCGCGTC900.095.000015
GAGTATA450.095.000012
CGCGCGT900.095.000014
CGCGCAC1050.095.000016
CGTCTAT500.095.000011
TCGTGTG1100.095.000018
CATACGA550.095.000018
CGTGTGC1100.095.000019
TGTAGTA450.095.000011
CACGTCG1100.095.000014
TGTCGCG1050.095.000013
TAGTAGT254.6009336E-795.000013
GATACGT156.6065934E-495.08
GATCGTC156.6065934E-495.05
GTCTCGA301.2245437E-895.07
CGTCTCG301.2245437E-895.06
TCTCGAC301.2245437E-895.08
CGAGCTC156.6065934E-495.01
Produced by FastQC (version 0.11.5)