FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC136CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC136CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040532
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGAGTACGATATATACTGAAACGAAAACGTTCTAATCTCTTGTTCC201771.93910422745288No Hit
CGTATGAGTACGATATATACTGATTCGAACTTTGAATTGTATGGGGACGG35540.3415560501743339No Hit
CGTATGAGTACGATATATACTGAAAAACCACCTTTCTTATTCTATTGCCT31490.30263365278530596No Hit
CGTATGAGTACGATATATACTGATCAGCAAAAGCAGGTCTCCCCATGCCT23240.22334728773358245No Hit
CGTATGAGTACGATATATACTGATTCAAAATGGAAGATTTTGTACGACAA22380.21508228483122097No Hit
CGTATGAGTACGATATATACTGTGGGGAGGGGCCAGGAGGCTGAGGGAGT15880.1526142396389539No Hit
CGTATGAGTACGATATATACTGGGGGACCTCCATTCACTACAACACAGAA13920.13377772139636263No Hit
CGTATGAGTACGATATATACTGAGAAGAGGTCAGATTGTAGAGATACTAT13790.1325283604925173No Hit
CGTATGAGTACGATATATACTGCCTAGCTTGCTTCCAGCATAGTTTGATT13650.13118289490376078No Hit
CGTATGAGTACGATATATACTGAAGACCCAGATGAAGGCACAGCTGGAGT12650.12157242641264276No Hit
CGTATGAGTACGATATATACTGAAGAAATCAGGAGCATATGGCATATATA12470.11984254208424153No Hit
CGTATGAGTACGATATATACTGTCTGGCTGTCAGTAAGTATGCTAGAGTC11230.1079255611552552No Hit
CGTATGAGTACGATATATACTGTGTGGGGAGGGGCCAGGAGGCTGAGGGA11020.10590736277212043No Hit
CGTATGAGTACGATATATACTGAAAAGCATGTGGCGATAGTCCTAGCTAC10790.10369695501916328No Hit
CGTATGAGTACGATATATACTGAGGCACCTGGGCCTCCTGCATTTGCTTG10440.10033329104727198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA823600.094.659731
GTATGAG827800.094.0761642
GAGTACG812350.093.713616
ATGAGTA817050.093.691954
AGTACGA811400.093.665277
TATGAGT819450.093.579843
GTACGAT811550.093.5484548
TGAGTAC816150.093.480975
TACGATA812050.093.2685859
CACGTCG2400.093.0208364
GTACGAA2100.088.214298
GTGTCGC2650.087.8301852
TCGCGCA2650.087.8301855
GGACGAT7200.086.4236158
GACGATA7200.086.4236159
CGTATAG2750.086.363641
GCGCGTC1550.085.806455
CGTATAA1500.085.499991
CGTGTCG3950.085.3797451
GAGGACG8100.084.444446
Produced by FastQC (version 0.11.5)