FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC134CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC134CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52014
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTATATAGCCCATCCACTGATAGAACAAAGAGATGAATTGCCAGCC2800.5383166070673281No Hit
CGTCGTATATAGTCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAAC1490.2864613373322567No Hit
CGTCGTATATAGTCTGTAGTCGCTCACCGGGCACGGTGAGCGTGAACACA1250.24031991386934287No Hit
CGTCGTATATAGTCTGTAGTCGGGGGTCACAGCCCCCATCCTGTTGTATA1020.19610104971738376No Hit
CGTCGTATATGAGTCCCATACAACTGGCAAGTGCACCAGCAGAATAACTG970.18648825316261006No Hit
CGTCGTATATAGTCAGCGAAATGCCCTGGGTAACATGTTCCATTCTCAGG860.1653401007421079No Hit
CGTCGTATATAGTCTGTAGTCGGCAATCTGTTCACAGGTTGCACATATAA810.1557273041873342No Hit
CACATACATTTGCCTCCCACCTGTCCCCCTAGGCTCACCTCTCCTGCACT760.14611450763256045No Hit
CGTCGTATATAGTCTGTATGAACGGGTCATGTTTCACCATAATGACCGAT710.13650171107778675No Hit
CGTCGTATATAGTCTGTAGTCGGTGGTCACAGCCCCCATCCTGTTGTATA660.126888914523013No Hit
CGTCGTATATAGTCAGTTAGGTACCGCGAAGCAAGTGCGGAGGCCATGGT610.11727611796823931No Hit
CGTCGTATATAGTCTTTAGCCACTCCATGAGAGCCTCAAGATCGGTATTC590.11343099934632984No Hit
CGTCGTATATAGTCTCTAGTCCCCAAGGCCATTAGAGGCCAATACAGTGG570.10958588072442034No Hit
CGTCGTATATAGTCATGTCAGTTAGGTACCGCGAAGCAAGTGCGGAGGCC560.1076633214134656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA409.094947E-1295.000014
GAGTATA409.094947E-1295.000012
GTCTACT201.7281476E-595.000016
GGATATA201.7281476E-595.000015
ACGATGT409.094947E-1295.000015
AGACGAT409.094947E-1295.000013
CGGATAT201.7281476E-595.000014
CGCTAGT201.7281476E-595.000011
ACGTCTA201.7281476E-595.000014
CGTGTAT409.094947E-1295.000011
CGCGTGT201.7281476E-595.000018
AGTACGA201.7281476E-595.000017
GAGTGTG301.2089004E-895.02
AGATCGT156.582139E-495.04
GATCGTC156.582139E-495.05
CGTATAC156.582139E-495.04
CGAGTGT301.2089004E-895.01
GCGCGTC301.2089004E-895.05
GAGTACG156.582139E-495.06
GTCTCAT156.582139E-495.09
Produced by FastQC (version 0.11.5)