FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC117CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC117CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149978
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTATCACTGAAGTTTTGTCCTCTCCAGCAACAACATTAGTGTTGTTAA3320.22136580031737987No Hit
CGCTATCACTACTATTTGCTTTCCAACACAAGTAGCTGTTTCGATGTTCA3290.219365506941018No Hit
CGCTATCACTACTAGTACTCTCGGGGGACTCGAACTGTGTTATCATTCCA2790.18602728400165358No Hit
CGCTATCACTACAACACAGAAACGGAAAATGGCGGGAACAACTAGGTCAG2790.18602728400165358No Hit
CGCTATCACTACTAGTACTCTCCGTCCCCATGCAACTGATTCGAACTTTG2670.17802611049620612No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA2190.14602141647441627No Hit
CGCTATCACTACTGGACTCTGCTTGAACCCGGGGATACAATAATATTTGA2040.1360199495926069No Hit
CGCTATCACTACTGAACTCAATTGCTCCCTCAGTTCCTCATAGTCAGCGA1980.13201936283988316No Hit
CGCTATCACTACTAGTACTCTCCAGCAACAACATTAGTGTTGTTAATATT1820.12135113149928656No Hit
GCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGCGAACA1640.10934937124111535No Hit
GACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA1610.10734907786475349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA301.2245437E-895.08
ACACGCG156.606612E-495.05
CGAGTAG156.606612E-495.01
CGCATAG301.2245437E-895.09
TCCCTAC156.606612E-495.06
ACGATGT950.095.05
GAGACGT1250.095.02
GTGTCGC1100.095.02
ACTACCC254.6009518E-795.08
CGTGTAT1150.095.01
CGATGTG950.095.06
CGTCGAT156.606612E-495.01
CGCGTCA1950.095.06
AGTAGAC156.606612E-495.03
GCGTCGA156.606612E-495.09
CACCACT156.606612E-495.07
ACGCGTC156.606612E-495.07
GACGTAC201.738867E-594.999995
TGACGTA201.738867E-594.999994
CTGACGT201.738867E-594.999993
Produced by FastQC (version 0.11.5)