FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC106CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC106CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311769
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATACGTCACGTGCTGTACCCCGACCAATCAGTTATTGCTACAACATCC5060.16229965134442487No Hit
GACTATACGTCACGTGCTGTACCCCGACCAATCAGTTATTGCTACAACAT4230.13567737651915363No Hit
ACTATACGTCACGTGCTGTACCCCGACCAATCAGTTATTGCTACAACATC3830.12284736455516745No Hit
CGACTATACGTCACGTGCTGTACCCCGACCAATCAGTTATTGCTACAACA3180.10199859511368994No Hit
CTATACGTCACGTGCTGTACCCTGACCAATCAGTTATTGCTACAACATCC3170.10167784481459029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG500.095.000016
TGTCGCG2000.092.625013
CACGTCG1500.091.833334
CTACTAC1250.091.28
CGCTATA3450.090.869571
CGCGCAC2150.090.58146
GTGTCGC2000.090.250012
GCGCGTC1550.088.8709645
CGATATA8400.088.779771
CACTATA8600.088.372091
AGACGTA700.088.214283
CGACATA1950.087.692311
TACGTCC1950.087.692317
CGACTAT38250.087.1764761
CGACTTA550.086.363631
CGCGTCA1600.086.093756
ACTATAG500.085.500013
CGATCGA500.085.500019
TGACTAT4900.085.3061141
CATACGA452.5465852E-1184.444448
Produced by FastQC (version 0.11.5)