FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC099CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC099CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332868
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT37601.129576889337515No Hit
CGTACGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACA26780.8045231142675175No Hit
CGTACGTCTACTACTCTACTGTATGAGCTACTTGGGAAGAATTTTTCAAA16850.506206664503647No Hit
CGTACGTCTACTACTCTCCTGTCTTCCTGATGTGTATTTCTTGATTATGG12600.3785284256822524No Hit
CGTACGTCTACTACTCTACTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC10840.3256546138409219No Hit
CGTACGTCTACTACTCTACTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG8900.2673732530612735No Hit
CGTACGTCTACTACTCTACTGTCTTCCCGATGTGTATTTCTTGATTATGG7950.23883341144237355No Hit
CGTACGTCTACTACTCTACTGTAGACCACATGGCCATAATCAAGAAATAC7560.22711705540935145No Hit
CGTACGTCTACTACTCTACTGTAGGCCACATGGCCATAATCAAGAAATAC7370.22140908708557144No Hit
CGTACGTCTACTACTCTACTGTCTTCCTGATGTGTATTTCTTGATTATGG6960.20909189228162517No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA4800.14420130502181044No Hit
GGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTG4730.14209836932357572No Hit
CGTACGTCTACTACTCTACTGTCTCCTCTGACTAAGGGCATTTTGGACAA4410.13248494898878835No Hit
GACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA4330.1300815939050915No Hit
CGTACGTCTACTATTTGCTTTCCAACACAAGTAGCTGTTTCGATGTTCAG4220.12677698066500834No Hit
CGTACGTCTACTACTCTACTGTCAGAAACGAATGGGGGTGCAGATGCAAC4030.12106901234122835No Hit
CGTACGTCTACTACTCTACTGTCGCCTCTGACTAAGGGCATTTTGGACAA3950.11866565725753152No Hit
CGTACGTCTACTGGACTCTGCTTGAACCCGGGGATACAATAATATTTGAG3790.11385894709013784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG254.613412E-795.01
ACGTTTA254.613412E-795.04
GCACGTC156.6137617E-495.02
GTAGGTC254.613412E-795.02
CGCTAGT254.613412E-795.01
GTCAACT156.6137617E-495.06
GGACGTC500.095.02
CGCGTCT156.6137617E-495.01
ACGAGTC156.6137617E-495.03
CGATCGA254.613412E-795.09
GCGTCGA254.613412E-795.09
CGTATAG2200.094.999991
GAACGTC201.7420027E-594.999992
ACGTCAT201.7420027E-594.999994
GCTACGT201.7420027E-594.999996
TAGATCG15950.094.106583
GTAGATC16400.092.682922
CACGTCG1800.092.361114
AGATCGT16000.092.3281254
ACGTCTT1350.091.4814764
Produced by FastQC (version 0.11.5)