FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC082CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC082CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302242
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT11230.37155656725405467No Hit
CGTAGATCGTCTCATACGTAGCCCCCCAAGATCAAAGGTCCCAGGTTCCA10450.34574943257389773No Hit
CGTAGATCGTCTTGTCTTTAGCCACTCCATGAGAGCCTCAAGATCGGTAT7760.25674790399745895No Hit
CGTAGATCGTCTCATACGTAGCCCCCGAAGATCAAAGGTCCCAGGTTCCA7420.24549864016251877No Hit
CGTAGATCGTCTCATACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAAT6680.22101494828647242No Hit
CGTAGATCCTCTCTACTATTTGCTTTCCAACACAAGTAGCTGTTTCGATG5800.19189920659603893No Hit
CGTAGATCGTCTCATACGTAGCAGGCACTGATTCAAAATGGAAGATTTTG5320.17601789294671158No Hit
CGTAGATCGTCTCATACGTAGCCGCCCAAGATCAAAGGTCCCAGGTTCCA5200.17204756453437975No Hit
CGTAGATCGTCTCATACGTAGCCCACCAAGATCAAAGGTCCCAGGTTCCA5110.16906981822513084No Hit
CGTAGATCGTCTCATACGTAGCCCCCTAAGATCAAAGGTCCCAGGTTCCA4990.165099489812799No Hit
GTAGATCGTCTCATACGTAGCCCCCCAAGATCAAAGGTCCCAGGTTCCAG4880.16146002210149482No Hit
CGTAGATCGTCTCATACAGAAGACCAGTTGGAATATCCAGTATGGTAGAG4860.1607983006994395No Hit
CGTAGATCGTCTCATACGTAGCCCCACAAGATCAAAGGTCCCAGGTTCCA4790.15848227579224594No Hit
CGTAGATCGTCTCATACGTAGCCCCCAAGATCAAAGGTCCCAGGTTCCAG4650.1538502259778588No Hit
CGTAGATCGTCTCATACGTAGCCTCCCAAGATCAAAGGTCCCAGGTTCCA4480.14822559406038868No Hit
CGTAGATCGTCTCATACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAAT4450.14723301195730573No Hit
CGTAGATCGTCTCATACGTAGCCACCCAAGATCAAAGGTCCCAGGTTCCA4140.13697633022544847No Hit
CGTAGATCGTCTCATACGTAGCCCTCCAAGATCAAAGGTCCCAGGTTCCA4080.13499116601928257No Hit
CGTAGATCGTCTCATACGTAGCACCCCAAGATCAAAGGTCCCAGGTTCCA3460.11447780255556805No Hit
CGTAGATCGTCTCATACGTAGCCCGCCAAGATCAAAGGTCCCAGGTTCCA3300.1091840313391256No Hit
GTAGATCGTCTCATACGTAGCCCCCGAAGATCAAAGGTCCCAGGTTCCAG3200.10587542432884908No Hit
CGTAGATCGGTATTCTTTCCAGCAAATACATCTTCAAGTCTCTGTGCGAT3140.10389026012268315No Hit
CGTAGATCGTCTCATACGTAGCAGAAAAAGCAATGAAAGAGTATGGAGAG3040.1005816531124066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT450.095.000014
CGTGTAT2350.095.000011
GCGCGTC1950.095.05
GAGTACA156.613167E-495.04
TATATCG301.2289092E-895.03
AGAGTAC156.613167E-495.03
ATATCGT254.612375E-795.04
CGTAGTT301.2289092E-895.01
CGTACAT254.612375E-795.01
CGTGTCG3100.095.01
TAGATGT156.613167E-495.03
CGTCGAT500.095.01
CGCGTCA1850.095.06
CAGAGTA156.613167E-495.08
CGATAGT301.2289092E-895.01
ACTATAC800.094.999993
CGTTAGA201.7417415E-594.999991
CGACTAG1600.094.999999
GACTATA800.094.999992
GTGTCGC2450.093.061222
Produced by FastQC (version 0.11.5)