FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC080CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC080CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44907
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC3330.7415325005010355No Hit
CGACGACGACACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTGCCAT810.18037277039214375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC201.7255597E-595.000015
GACGTAC201.7255597E-595.000015
CGCGCGT201.7255597E-595.000014
CGCGCAC201.7255597E-595.000016
CGACATA409.094947E-1295.000011
TGACGTA201.7255597E-595.000014
CTGACGT201.7255597E-595.000013
GTAGAGT201.7255597E-595.000012
CGCTAGA201.7255597E-595.000011
ACGCTGT201.7255597E-595.000017
TCGTACT201.7255597E-595.000017
ATAGTCG201.7255597E-595.000013
GCGTCAC201.7255597E-595.000017
TCGCGCG201.7255597E-595.000013
AGTCGTA201.7255597E-595.000015
TATCATA254.5482375E-795.05
CGTCTCG353.2014214E-1095.06
GTGTCGT353.2014214E-1095.02
GTGTCGC254.5482375E-795.02
TCTCGAC353.2014214E-1095.08
Produced by FastQC (version 0.11.5)