FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC078CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC078CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24625
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCCCTCTATTATCTCAAATCTGTGCTTTAAAAGTGCATTTGGGTCCTC1420.5766497461928934No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAGAGAACGTACGTTTCGACC1200.4873096446700508No Hit
CGACGACGGAGCTTCACCTAGAGGACAGCTCATTAAGGCCCTATAAGGAC270.10964467005076142No Hit
CGTCGTGCTGCTACGTCGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGA260.10558375634517765Illumina Single End PCR Primer 1 (100% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTA850.095.000013
CGTGTCG450.095.000011
GAGACGT850.095.000012
GTAGAGT301.1823431E-895.02
GTATGAG254.4867375E-795.02
GTGTATA156.5405364E-495.02
ACGTAGA500.095.05
CGTAGAG301.1823431E-895.01
GAGTATA156.5405364E-495.02
ATACGAC156.5405364E-495.09
AGATGAC500.095.09
GTCTATC156.5405364E-495.02
CGTGTAT156.5405364E-495.01
CGTCGTA156.5405364E-495.01
ACGATGT156.5405364E-495.05
CGAGACG1000.095.01
CATACGA156.5405364E-495.08
TAGATGA500.095.08
CTACTAG156.5405364E-495.09
CTACTAC156.5405364E-495.08
Produced by FastQC (version 0.11.5)