FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC070CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC070CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92229
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGAGCTACGTCAATGCAGCAGCCTACGGGGAAATGAAGCAGGGGGCTG2350.25480055080289277No Hit
CGTGAGCTACGGGAACAGATTGATAACGTTTACTAAACAGACCCAGACTT1730.18757657569744873No Hit
CGTGAGCTACGCTGTGTGTGTGCATGTGCGGGTGTGTGAGAGTGTGTGTG1540.1669756801006191No Hit
CCCATCCTGAAGCAGGTCACATCAGCCTCAAACGTCATCCAGAGGACCAC1240.13444795021088812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG254.5868E-795.000016
CTACACG254.5868E-795.000013
GCGTCGA254.5868E-795.000019
GTCGCGT301.2194505E-895.06
AGATCGT156.5984693E-495.04
GCGCGTC156.5984693E-495.05
TAGACGT301.2194505E-895.09
GTCTATC156.5984693E-495.02
GAGACGA156.5984693E-495.02
ATGAGCT156.5984693E-495.02
GTGTCGT450.095.02
ACTACGA450.095.05
CATACGA156.5984693E-495.08
CTCGCAC156.5984693E-495.09
TACTCGC156.5984693E-495.07
CGTGTCG600.095.01
TAGATCG156.5984693E-495.03
CACGTCG450.095.04
CGCGTCA156.5984693E-495.06
TGTCGCG156.5984693E-495.03
Produced by FastQC (version 0.11.5)