FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC062CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC062CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90457
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC5500.6080237018693966No Hit
CGACGAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAACGCG2380.26310843826348435No Hit
ACGCGTGATCAGTAGAAACAAGGTATTTTTTCATGAAGGACAAGTTAAAT1420.15698066484628057No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1180.1304487214919796No Hit
CGACGAGTCACCCAGGGGAGTGGAGGAAGGCTCTATTGGGAAAGTGTGCA1170.12934322385221708No Hit
CAGGAAAGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATT1130.12492123329316691No Hit
CACCATGGACACAGTGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATG1060.11718274981482914No Hit
CAGCAAATATCATTGGGATCTTGCACCTGATATTGTGGATTACTGATCGT1060.11718274981482914No Hit
CGACGAGTCTCTGTGTTTGTCGTCCACTTTCCCTTTTCTGAGTATTGGTG1000.11054976397625392No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT950.10502227577744122No Hit
GGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACG910.10060028521839107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA201.7351182E-595.000016
TCGCATA201.7351182E-595.000018
GTACACT409.094947E-1295.000018
GCACGCG201.7351182E-595.000017
CGACAGC201.7351182E-595.000014
GAGACGT409.094947E-1295.000012
TCTCGCA201.7351182E-595.000016
CTCGCAT201.7351182E-595.000017
TATACGA201.7351182E-595.000014
GTGACGT201.7351182E-595.000014
ACATACA409.094947E-1295.000013
AGTCGCA201.7351182E-595.000013
GCGAGTC201.7351182E-595.000019
GTCGATA353.255991E-1095.02
CTATAGT254.5860907E-795.09
GAGTACA950.095.04
AGACGTA353.255991E-1095.03
AGAGTAC950.095.03
GTAGATG353.255991E-1095.07
CTAGTCG254.5860907E-795.03
Produced by FastQC (version 0.11.5)