FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC060CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC060CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8010
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAGTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCC871.0861423220973783No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC150.18726591760299627No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC140.17478152309612985No Hit
CGCGAGCGAGCATGCGTGTACACACACGTGTGCATGTGGCAGGGCAGCAG130.16229712858926343No Hit
CGCGAGCGATTCAAGTGATCCTCTCGTCATTGCAGCAAATATCATTGGGA110.1373283395755306No Hit
CGCGAGCAAGCCACATCTCGGGACCCCTTCTTGGAAAACTCGCAAACCCA110.1373283395755306No Hit
ACGCGTGATCAGTAGAAACAAGGCTATGGAAGCTTCAGATCCTGGGAGGG110.1373283395755306No Hit
CGCGAGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTAAT100.12484394506866417No Hit
CGACAACCAACTTCAATTCTACCAGCAGATAAAAAGCCTGATCAAGGTAA90.11235955056179776No Hit
CGCGGGTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCC90.11235955056179776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACA201.6396923E-595.000016
CTCTATA201.6396923E-595.000018
TCTATAC201.6396923E-595.000019
TCAGTCT201.6396923E-595.000013
AGAGTAC201.6396923E-595.000013
GAGTACA201.6396923E-595.000014
GTCTCTA201.6396923E-595.000016
TCTCTAT201.6396923E-595.000017
CGAGAGT201.6396923E-595.000011
GTCAGTC201.6396923E-595.000012
CGCGAGC201.6396923E-595.000011
CGACTAT156.378235E-495.01
GCGAGCG156.378235E-495.02
GACTATA156.378235E-495.02
CTCGACG452.0008883E-1184.444449
GTGTCGT452.0008883E-1184.444442
GTCGTCT452.0008883E-1184.444444
TCTCGAC452.0008883E-1184.444448
GTCTCGA452.0008883E-1184.444447
TCGTCTC452.0008883E-1184.444445
Produced by FastQC (version 0.11.5)