FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC059CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC059CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAGCGAGACAGTCGTGTCGGGCACAACGACAGCTGCGTCGACTACGC22.380952380952381No Hit
CGCGAGCGCGACAGTCGTGGCAGACCCGACAGCTGCGTCGACTAAACACA11.1904761904761905No Hit
CCGAGCGAGACAGTCGTGTCGCCCCCCGACAGCTGCGTCGACTACGCGAG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGAGGAAAGGGCATGATCAACTGGA11.1904761904761905No Hit
CGTGAGCGCGACAGGCGTATCGGAACAAAACCTGCGGCGACTACGACAGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAGGGGCCGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAAGGAACGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGTGGCACTACCATTAGCTCCTGGAAGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCAAGGGCAAACGACAGCTGCGTCGACT11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGTCCGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGAACCCGACAGCTGCGTCGACTACGCGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGGGCGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCCACACGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCGCCCCGACAGCTGCGTCGACTACGCGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGAAAGGGAACGAAAACGGGACTCTAGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAAGAGGCGACAGCTGCGCCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCTTGTCGGACACCCCGACAGCTGCGTTGACTACGC11.1904761904761905No Hit
CGAGATGAGTCTATATACGTCGAGGCAAAGAAGACAAGAGATATGGCCCA11.1904761904761905No Hit
CGAGATCGCGAGAGGCGCGACGGTGCCCCGAATGCTGCGACGACAAGGGG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGATGAAACGAAAACGGGACTCTAGCATAC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGACGGAGAAGAGCGTGCTTGAGGACAG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCCCCCGACAGCTGCGTCGACTTCGCGG11.1904761904761905No Hit
GCGAAGGAGACAAACGGGTCGCGGGCCCCCCACAACCGCGGCCCCTACGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCAAAGGAGAAAAGCTAATGTGCTAATTG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGCAGGAGAAGAGGAAGGTGGGATGAGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAGGGGCCCAACGACAGCTGCGTCGACTA11.1904761904761905No Hit
CGCGAGCGTGACAGTGGTGTCGCGCCCCCCCGACAGCTGCGGCGACTAGG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGACGCGCCACGACAGCTGCGTCGCCTACGCGA11.1904761904761905No Hit
CGCGAGCGAGACAGCCGTGTGGCCAGGCAGAATCAAGAACCCATGGGACA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCAGGGGAGAAAATGGACGAAGGACAAGG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCCCCCCGACAGCTGCGCCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAACCGACAGCTGCGGCGACTATGCGAGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGAAACGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGAAGGACAAGTTAAATTCATTATTTTT11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGCACAGAAATTTGAGGAAATAAGGTGG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGACCGACAGCTGCGTCGACTACGCGAG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCGTCCACCCGACAGCTGCGTCGACTACG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGCCACGACAGCTGCGTCGACTACGCGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAGAGAAAGCTAATGTGCTAATTGGGCAA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGAGGCAAAACTGTTTTATGGTGTCAG11.1904761904761905No Hit
GGAGGACTTGAATGGAATGGTAACACGGTTCGAGTCTCTGAAAGTATACA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGCCGGAAACGACAGCTGCGGAGACTA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGACGGAAATTTGAGGAAATAAGGTG11.1904761904761905No Hit
GAGAGCGGGACAGGGGTGGGGGCCAGGGGGGGGGGGGTTGGCGGGGGGAC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGCCGGCCGACAGCTGCGTCGACTACGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGGGGGAACGCGACAGCAGCGCCGAC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGAGGGAGAAAAAAATTGGACAAATTGG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGAGATCACTTGGTGAGAGAGAAAAC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCGCCACCCGACAGCTGCGTCGACTACGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCGGACAGAAATTTGAGGAAATAAGGTG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCAACCACCACACCAACGACAACCACAC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCAGCCCGGAACGACAGCTGCGTCGACTA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCCCCCCCGACAGCTGCGTCGACTACGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAAAGGCCGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCAAAAGGAAGAGATATGGCCAGGAGAC11.1904761904761905No Hit
CGCCGGCGAAACACTCGGGGCGGCCCCGACAGTTTTTGGGGGGACCCCTT11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCAACAAAAAGCAAAGCCTTTTATAAAA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGAGGAGAGAAGAAAAATATTTTTGGATA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGTGACCCGACAGCTGCGTCGACTACGCGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGAAGGGAACGAAAACGGGACTCTAGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCCCCCGACAGCTGCGTCGACTACGCGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGAGGGCCAAAACGACAGCTGCGGCGACT11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAAAAGAACTGAGAGATCTAATGTCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCGGGGGGAACAGAAGGAAGAATCCAGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGATCAGCAAAAGCAGGAGCAACCCAGAGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCCGACCGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGAGCCCCCGACAGCTGCGTCGACTACGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCCGACAGCTGCGTCGACTACGCGAGAT11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCAGGCCCACGACAGCTGCGTCGACTAC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGCCCACGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCCAACCGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCCCGACAGCTGCGTCGACTACGCGAGA11.1904761904761905No Hit
CGTGAGCTACGTCGTGCACGTAATTGCCCACGCGTGATCAGTAGAAACAA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCCCCACGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGAAACCCGACAGCTGCGTCGACTACGCGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGCCCCGACAGCTGCGGCGCCTACGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCCCAACGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCGGGGACGACAGCTGCGTCGACTACGC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGACCGACAGCTGCGTCGACTGCGCGAGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGGCCAACCGACAGCTGCGGCGACTACGCG11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCCCCGACAGCTGAGTCGACTACGCGAGA11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGACCCAACAAAGTAGGCATCTCGGTCATC11.1904761904761905No Hit
CGCGAGCGAGACAGTCGTGTCGCGGCCCCGACAGCTGCGTCGACTACGCG11.1904761904761905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAG50.094.999994
CGAGCGA50.094.999993
CGAGACA50.094.999997
AGCGAGA50.094.999995
AGACAGT50.094.999999
GCGAGCG50.094.999992
GCGAGAC50.094.999996
GAGACAG50.094.999998
CGCGAGC50.094.999991
Produced by FastQC (version 0.11.5)