FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC050CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC050CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99558
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGTACACACTACTAACCATTAATCTTGCGTAGCAGAGGCTCGAGTT2850.2862652925932622No Hit
CGAGAGTACACACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAAT2110.21193676048132745No Hit
CGAGAGTACACACACACAATACACACATATAAACGTATTCTTATATGTTC1840.18481689065670262No Hit
CGAGAGTACACTACTAACCATTAATCTTGCGTAGCAGAGGCTCGAGTTTT1400.1406215472387955No Hit
ACGCGTGATCAGCAAAAGCAGGTTGATGTGCTAAATGTCAATTATTTCAA1130.11350167741417064No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC1120.11249723779103638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATC800.095.000012
CACGTCG201.7359811E-595.000014
CGTATAG254.5895104E-795.01
CGAGTAG600.095.01
AGATACG156.600029E-495.07
GCGCGTC301.22036E-895.05
TAGACGT156.600029E-495.09
CGCGCGT301.22036E-895.04
CGACATA600.095.01
CGCTGAC254.5895104E-795.01
CGTAGTA254.5895104E-795.01
CGCTATC156.600029E-495.01
CGAGGTA254.5895104E-795.01
CTACGAC650.095.06
CGCTACA301.22036E-895.01
CGACTGC550.095.09
CGACTAG254.5895104E-795.09
GCGTCGA254.5895104E-795.09
CGGAGTA500.095.01
TCGCGCG301.22036E-895.03
Produced by FastQC (version 0.11.5)