FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC047CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC047CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36063
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGC409.094947E-1295.000011
AGAGTAC201.7209131E-595.000013
CGAGATG201.7209131E-595.000011
CGATGTG201.7209131E-595.000016
CGAGAGT201.7209131E-595.000011
TATCATA254.529884E-795.05
CGAGTAG353.1832315E-1095.01
GAGTATA600.095.02
GTCTCGA156.565594E-495.07
GTCTATC254.529884E-795.02
CGTCTCG156.565594E-495.06
CGTCTAT254.529884E-795.01
CGACATA254.529884E-795.01
CTACGAC550.095.06
GTGTCGT156.565594E-495.02
ACTACGA600.095.05
GTCGTCT156.565594E-495.04
GTGTACA156.565594E-495.09
CGTACGC254.529884E-795.04
CGTCGTA301.1981683E-895.01
Produced by FastQC (version 0.11.5)