FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC046CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC046CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130320
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACA2960.22713321055862495No Hit
CGAGACTACTGCAAGCCCATACACACAAGCAGGCAGGCAGGATTTATGTG2770.21255371393492942No Hit
CGAGACTACGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGA2350.18032535297728666No Hit
CGAGACTACGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCG2100.16114180478821363No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC1630.1250767341927563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAC201.7380067E-595.000013
GAGACGT409.094947E-1295.000012
CGCTATC201.7380067E-595.000011
GCTACGT201.7380067E-595.000016
CGACTAT201.7380067E-595.000011
TATCATA1800.095.05
CTCACGC500.095.08
CGAACTA450.095.01
AGATCGT156.60465E-495.04
GTCGATA353.274181E-1095.02
CTATAGT254.5975503E-795.09
CGTGACT450.095.01
CGCGCGT301.2232704E-895.04
CGCGCAC500.095.06
GCTGACG650.095.02
ACGATGT500.095.05
TCGTGTG353.274181E-1095.08
CGCTCAT254.5975503E-795.01
CACTACT156.60465E-495.07
CGCTACA353.274181E-1095.01
Produced by FastQC (version 0.11.5)