FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC045CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC045CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173319
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCGTTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCAC9620.555045898026183No Hit
CGTGTCGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATA7800.45003721461582397No Hit
CGTGTCGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGTTT5350.30867937156341774No Hit
CGTGTCGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGAAAT4530.26136776694995933No Hit
CGTGTCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCGAGAAAATTGC4430.2555980590702693No Hit
CGTGTCGTCCATCATAATCACTGAGTTTGAGTTCAGTGCACATTTGGATG3020.17424517796663955No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC2530.1459736093561583No Hit
CGTGTCGTCTGAATTTGTGTGTCTCCCCTATGGCACCTATATGTGTATCT2460.14193481384037526No Hit
CGTGTCGTCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA1760.1015468586825449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCG156.608365E-495.01
GTCGCAC301.225817E-895.04
CGAGTAG2050.095.01
CTATCAC301.225817E-895.03
GCTATCA301.225817E-895.02
ACGATGT450.095.05
TCGTCCC254.6040077E-795.05
GTCGTCA450.095.04
CTCGCCG156.608365E-495.09
CTCGCAT301.225817E-895.07
CGAGATG1650.095.01
CGAGAGT301.225817E-895.01
CGATAGT850.095.01
TACGTCA301.225817E-895.07
GTCGATA550.094.999992
CTATAGT353.274181E-1094.999999
ATGTCGT201.7396353E-594.999992
TCGGCGT201.7396353E-594.999999
GAGACGT550.094.999992
CGTCGTA1150.094.999991
Produced by FastQC (version 0.11.5)