FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC044CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC044CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138404
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGACATCCGACATGCGAACAGAGGTTATAAGAATGATGGAAAGTG3400.24565763995260254No Hit
CGAGTAGACGATCTCGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAA3370.24349007254125601No Hit
CGAGTAGACAATCCACGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGT2730.19724863443253085No Hit
CGAGTAGACGAGCAGTCAATTGCTGAGGCAATAATTGTGGCCATGGTATT2500.18063061761220772No Hit
CGAGTAGACTTTGATGACTGCAAAGATGTTGGAGACCTTAAACAGTATGA2230.1611225109100893No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC1970.1423369266784197No Hit
CGAGTAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCA1740.12571890985809658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAG409.094947E-1295.000011
CGTGACG409.094947E-1295.000013
GCTATCA201.7383909E-595.000012
CGCGCGT450.095.000014
CGCGCAC409.094947E-1295.000016
GCGCACT409.094947E-1295.000017
CTACGAC850.095.000016
CACTACT201.7383909E-595.000017
ACTACGA850.095.000015
CGTCGTA900.095.000011
CGCGTCA450.095.000016
ACATACA950.095.000013
TGTCGCG409.094947E-1295.000013
CTCACGC700.095.08
CGTATAG750.095.01
CGAGTAA353.274181E-1095.01
CTATAGT254.5990782E-795.09
CGTGAGC254.5990782E-795.01
CATAGTC254.5990782E-795.03
CTAGTCG156.6055276E-495.03
Produced by FastQC (version 0.11.5)