FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC035CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC035CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131189
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGACGGACATCCGACATGCGAACAGAGGTTATAAGAATGATGGAA5000.3811295154319341No Hit
CGAGATGACGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGA3990.3041413533146834No Hit
CGAGATGACGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTA3750.28584713657395056No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC2960.22562867313570498No Hit
CGAGATGACCGAAATTTCTGGAGGGGTGAAAATGGACGAAGGACAAGGGT2720.20733445639497217No Hit
CGAGATGACGACACCCACGGTCGATTCGAGCCATGCCAGTTATCCCTGCA2310.17608183612955355No Hit
GATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT1880.14330469780240723No Hit
CGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAGT1850.14101792070981561No Hit
CGAGATGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG1740.13263307137031305No Hit
CGAGATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA1710.13034629427772146No Hit
AGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTA1620.12348596299994664No Hit
CGAGATGACGAGACATCCGACATGCGAACAGAGGTTATAAGAATGATGGA1590.12119918590735503No Hit
CGAGATGACGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGG1370.10442948722834994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA201.7380493E-595.000018
ACTCTCG201.7380493E-595.000014
TCGTGTG409.094947E-1295.000018
CGGATGA1050.095.000011
TCTCGCA201.7380493E-595.000016
CTCGCAT201.7380493E-595.000017
AGTCGCA409.094947E-1295.000013
CTCACGC600.095.08
CGTATAG450.095.01
TAGACGT600.095.09
ATACTCG156.604747E-495.06
AGAGTAC600.095.03
GTATGTA750.095.02
CGAGGTG156.604747E-495.01
CTACGAC1450.095.06
ACTACGA1450.095.05
GTGTATA301.2234523E-895.02
ATGACAT254.597714E-795.05
CGTACGC1150.095.04
TACTCGC156.604747E-495.07
Produced by FastQC (version 0.11.5)