FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC026CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC026CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59411
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTCTAGATACTCTGTAGAGCCCCGATTGGGCCAAAAGAACCAAAACC1820.3063405766608877No Hit
CCAAAAGAACCAAAACCAAAGGCCTTAATCCCATTATATTTCTTGAAGAA950.15990304825705676No Hit
ACGCGTGATCAGCAAAAGCAGGGCCAAACTGCCTCATCACGGGACTGTCT850.1430711484405245No Hit
ACGCGTGATCAGCAAAAGCAGGCATGTATGTCCAGCATAAACCTGCAGAG750.1262392486239922No Hit
CGCGTCTAGATACTCTGTAGAGAAAAACCAAAGTTTAATGATATGGGTCC650.1094073488074599No Hit
CGCGTCTAGATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATG640.10772415882580666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACTC409.094947E-1295.000019
CGTGTCG201.7301882E-595.000011
CGTCGTA201.7301882E-595.000011
CACGTCT409.094947E-1295.000011
CGATCGA201.7301882E-595.000019
GATACTT254.5665547E-795.09
CGCGGCC156.586802E-495.01
TAGACGA254.5665547E-795.05
GTCTATA156.586802E-495.04
CATCTAG301.2118107E-895.03
CGTCTAA156.586802E-495.03
TCTACTA156.586802E-495.07
CATACGA254.5665547E-795.08
TAGATAG156.586802E-495.07
CTACACG254.5665547E-795.03
ACTCACG156.586802E-495.07
TACACGC254.5665547E-795.04
ACGCGTC254.5665547E-795.07
GCTACGT353.237801E-1094.999996
AGCTACG353.237801E-1094.999995
Produced by FastQC (version 0.11.5)