FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC025CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC025CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301476
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT15990.5303904788440871No Hit
CGTCTATCATCCGACATGCGAACAGAGGTTATAAGAATGATGGAAAGTGC12620.4186071196380475No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT11350.3764810465841394No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGGGTTTT9300.3084822672451539No Hit
CGTCTATCATACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCC8540.28327296368533483No Hit
CGTCTATCATACAACTACTGCTTGAAGTAGAACAAGAGATAAGAGCTTTC6680.22157651023630406No Hit
CGTCTATCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCC5820.19305019305019305No Hit
CGTCTATCATACGACTGTCTACATTCCCATTTAGGGCATTTTGGATAAAG5460.18110894399554195No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA4990.16551897995196962No Hit
GGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACG4670.1549045363478353No Hit
CGTCTATCATACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGA4510.14959731454576816No Hit
GGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTG4510.14959731454576816No Hit
CGTCTATCATACGACATCCGACATGCGAACAGAGGTTATAAGAATGATGG3780.12538311507383673No Hit
CGTCTATCATATGTCTGATTTACCCAAGTGTTGTTTTCATAAGTAATGAC3410.11311016465655642No Hit
CAGATGCAGCGATTCAAGTGATCCTCTCGTCATTGCAGCAAATATCATTG3310.10979315103026442No Hit
GTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTTT3260.10813464421711844No Hit
TGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGTCTT3130.10382252650293887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAC500.095.000014
GTACACT409.094947E-1295.000018
CGCCTAT201.7417344E-595.000011
TCTCGCA409.094947E-1295.000016
TGAGCTA500.095.000013
CGATAGC201.7417344E-595.000018
ATACACG201.7417344E-595.000018
CTATCGT550.095.04
TCATTCG650.095.07
CGCGCAC700.095.06
CGTCTCG1400.095.06
ATCAAAT156.613151E-495.06
ACGATGT1100.095.05
TCGTGTG550.095.08
CTAGTCG650.095.03
ACTCGCA301.2289092E-895.08
CTCGCAC301.2289092E-895.09
CGATGTG1150.095.06
GCTACAC1700.095.02
GATAGTA156.613151E-495.08
Produced by FastQC (version 0.11.5)