FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC019CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC019CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249123
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGGCATCACTGTCACAGTGGTGAGTGAT23320.9360837819069295No Hit
ACGCGTGATCAGTAGAAACAAGGGGTGTTTTCTGTTAGATGCCAGGAGGT9820.3941827932386813No Hit
ACGCGTGATCAGTAGAAACAAGGGCCCTTCATGTTTCTCACACTCCCTTG6540.26252092339928473No Hit
ACGCGTGATCAGTAGAAACAAGGACTGTAGCAGCTACATTTTATGCTAAT5420.21756321174680782No Hit
CGAGCTCTATACGTGTAGTCTCTATGTTGACAAAATGACCATCGTCAACA4830.19388013150130656No Hit
ACGCGTGATCAGCAAAAGCAGGCCCTGTACATGGAATGCTATCCTCTGTG4000.16056325590170317No Hit
ACGCGTGATCAGCAAAAGCAGGAATCACTCTAGAATTATGCCTTTGACAT3930.15775339892342338No Hit
ACGCGTGATCAGTAGAAACAAGGGTGAGAAGATGTAGGAGCTGTCCACCC3600.14450693031153286No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTGAACTTTGTTTTCTTGATTTGAA2930.11761258494799758No Hit
CGAGCTCTATACGTGTAGTCTCCTTCCTCCCTCATCCCCACTTCACTGAA2660.10677456517463262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC201.7411374E-595.000014
GTGTCGT800.095.000012
CGGTATA409.094947E-1295.000011
ATTCGTA750.095.06
CGCTACA450.095.01
CGAATAT254.609974E-795.01
CGTCGTA1950.095.01
CGAGAAT353.2923708E-1095.01
CGTCCGT156.611789E-495.09
CGAGTAT52700.093.648011
CGAGCTC57550.089.3049551
GTATTCG800.089.062514
TTCGTAC800.089.062517
CATACGA1600.089.062518
CGAGATG700.088.214291
CGTGTCG1850.087.29731
CACGTCG600.087.0833364
GAGCTCT59900.085.880642
GAGTATA57650.085.8542942
ATACGAC1900.085.09
Produced by FastQC (version 0.11.5)