FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC015CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC015CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118676
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAA9230.7777478175873808No Hit
CGTCGTACGTTTCGACCTCGGTTAGAAGACTCATCTTTAAATATCTACCT1720.14493242104553575No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC1610.1356634871414608No Hit
CGTCGTACGCTGTTTCGATATCGAGGCCAAGGGTGTTGCCTCTTCCTTTT1350.11375509791364724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAC450.095.01
TAGACGT1050.095.09
CTATAGT254.5949855E-795.09
GCTATAC156.603182E-495.02
CGCGCGT600.095.04
GTCTAGA254.5949855E-795.04
CGCTGAT156.603182E-495.08
ACGATGT156.603182E-495.05
TCGTGTG254.5949855E-795.08
ACGATAG156.603182E-495.07
TATCTAT254.5949855E-795.06
CATAGTC156.603182E-495.03
CATACGA650.095.08
CTACACG450.095.03
CGAGATG1650.095.01
GCTACAC450.095.02
CACGTCG254.5949855E-795.04
ATACGAT156.603182E-495.03
CGACTAG301.2223609E-895.09
GACGACG156.603182E-495.02
Produced by FastQC (version 0.11.5)