FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC009CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC009CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155867
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTG3770.24187287880051583No Hit
GGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACG2760.17707404389639886No Hit
CGAGCTCTATACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACC2750.17643247127358583No Hit
CGAGTATACGTACGTCTCAGTCCAGGAGGGAAGAATATCAACAGGAACAG2520.1616763009488859No Hit
CGAGTATACGTACGTCTCAGTCCGGGGGAGAATATCAACAGGAACAGCAG2450.15718529258919464No Hit
CGAGTATACGTACGTCTCAGTCCGGGGAAGAATATCAACAGGAACAGCAG2400.15397742947512943No Hit
GGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTG2170.13922125915042952No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA1900.12189879833447746No Hit
CGAGTATACGTACGTCTCAGTCGAGGGGAGAATATCAACAGGAACAGCAG1690.10842577325540365No Hit
CGAGTATACGTACGTCTCAGTCAGGGGAAGAATATCAACAGGAACAGCAG1640.10521791014133845No Hit
TGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGT1600.1026516196500863No Hit
CGAGTATACGTACGTCTCAGTCAAGGAGGGAAGAATATCAACAGGAACAG1570.10072690178164717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG156.607104E-495.07
GTATTCG156.607104E-495.04
ATTCGTA156.607104E-495.06
TTCGTAC156.607104E-495.07
ATCCGTG156.607104E-495.09
GAGACGT156.607104E-495.02
CGCTACA353.274181E-1095.01
GCCCTAT156.607104E-495.04
ACGGACG156.607104E-495.08
CGCACGT156.607104E-495.09
ATAGTCG301.2249075E-895.03
CACGTCG301.2249075E-895.04
CGACTGC301.2249075E-895.09
GCGTCAC301.2249075E-895.07
CGGACGT156.607104E-495.09
CTATACT201.7390828E-594.999997
CGCTGAC201.7390828E-594.999991
CGACTAG201.7390828E-594.999999
AGCTACG201.7390828E-594.999995
TGAGCTA201.7390828E-594.999993
Produced by FastQC (version 0.11.5)