FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6937238
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGCGTGTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC152980.22052003981988222TruSeq Adapter, Index 5 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG117960.1700388540799667TruSeq Adapter, Index 5 (100% over 49bp)
CGCTACACGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC92820.13379964764074695TruSeq Adapter, Index 5 (100% over 35bp)
CGAGTCGAGTGCGCGACACGAGATCGGAAGAGCACACGTCTGAACTCCAG79760.11497371143962481Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC71170.10259126182495107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACA13350.074.181131
TGTAGCG25650.067.035468
GACTATA56950.066.1293261
GTGTAGC26400.065.6703347
GTGTCGT15900.063.4819221
CGCGTGA337650.062.725132
GCGTGAT342050.061.8627173
GCTACAC16300.060.6103252
ACGCGTG359000.059.1098061
CGTGATC359900.058.5173574
GTAGCGA29750.058.275949
GTAGATC44750.058.0912781
GTGATCA364750.057.4930955
GCGTGTA30700.057.2457965
TGATCAG371800.056.2112926
GACGCGT31750.055.5010342
GTGCGCG16600.055.2246069
CACTATA21950.054.6609731
GATCAGC185800.054.5798657
AGTGCGC16950.054.3645028
Produced by FastQC (version 0.11.5)