FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC379CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC379CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164605
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT97275.909298016463655No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGTAGTTTT21561.3098022538805019No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTATTTT20301.2332553689134595No Hit
CGAGACGATGTGTACGTACTACCCGGGAACAACTAGGTCAGAAGTTTGAA16751.017587558093618No Hit
CGAGACGATGTGTACGTACTACCCGCGCTGGGATGTTCCTCTAGATATTT13940.8468758543179127No Hit
CGAGACGATGTGTACGTACTACGGGGCGGGAACAACTAGGTCAGAAGTTT11880.721727772546399No Hit
CGAGACGATGTGTACGTACTACGGGGGCAGAAGTTTGAAGAAGTAAGATG10250.6227028340572887No Hit
CGAGACGATGTGTACGTACTACGGGGGAACAACTAGGTCAGAAGTTTGAA10200.6196652592570092No Hit
CGAGACGATGTGTACGTACTACCGGGGCAGAAGTTTGAAGAAGTAAGATG10050.6105525348561709No Hit
CGAGACGATGTGTACGTACTACAAGGGCAGAAGTTTGAAGAAGTAAGATG8930.542510859329911No Hit
CGAGACGATGTGTACGTACTACCGGGCGGGAACAACTAGGTCAGAAGTTT8850.5376507396494639No Hit
CGAGACGATGTGTACGTACTACAGGGCGGGAACAACTAGGTCAGAAGTTT8500.5163877160475077No Hit
CGAGACGATGTGTACGTACTACAAGGGAACAACTAGGTCAGAAGTTTGAA8360.5078825066067252No Hit
CGAGACGATGTGTACGTACTACGAGGGCAGAAGTTTGAAGAAGTAAGATG7420.4507761003614714No Hit
CGAGACGATGTGTACGTACTACGGGGGGGGAAGTCTTTGAGCTCTCGGAC7130.43315816651985056No Hit
CGAGACGATGTGTACGTACTACGGGGGGAGAATGGAAGAAAAACAAGGAT7010.4258679869991799No Hit
CGAGACGATGTGTACGTACTACCAGGGCAGAAGTTTGAAGAAGTAAGATG6590.40035235867683244No Hit
CGAGACGATGTGTACGTACTACGGGGGGGGAACAACTAGGTCAGAAGTTT6090.36997661067403786No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGTCGTTTT5700.34628352723185807No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGGGTTTT5680.3450684973117463No Hit
CGAGACGATGTGTACGTACTACCAGGCGGGAACAACTAGGTCAGAAGTTT5320.3231979587497342No Hit
CGAGACGATGTGTACGTACTACAGGGGCAGAAGTTTGAAGAAGTAAGATG4840.29403724066705145No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCAAAAGCAGGTAGATATT4730.28735457610643667No Hit
CGAGACGATGTGTACGTACTACAAGGCGGGAACAACTAGGTCAGAAGTTT4420.26852161234470395No Hit
CGAGACGATGTGTACGTACTACCCGGGGACAACTAGGTCAGAAGTTTGAA4280.2600164029039215No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCGAAAGCAGGTAGATATT4220.2563713131435862No Hit
CGAGACGATGTGTACGTACTACAGGCAAATGGTAACAACAACCAATCCAT4220.2563713131435862No Hit
CGAGACGATGTGTACGTACTACTAGGTCAGAAGTTTGAAGAAGTAAGATG4100.24908113362291545No Hit
CGAGACGATGTGTACGTACTACGAGGGAACAACTAGGTCAGAAGTTTGAA4030.2448285289025242No Hit
CGAGACGATGTGTACGTACTACCGGAGAAGGCCCTCTTTTTAAACCGTGT3880.23571580450168586No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCAAAAGCAGGGTAGATAA3630.2205279305002886No Hit
CGAGACGATGTGTACGTACTACCCGGCGGGAACAACTAGGTCAGAAGTTT3510.2132377509796179No Hit
CGAGACGATGTGTACGTACTACTGGGCCATAAGAACCAGAAGTGGAGGGA3490.2120227210595061No Hit
CGAGACGATGTGTACGTACTACCCGGGCAGAAGTTTGAAGAAGTAAGATG3450.20959266121928252No Hit
CGAGACGATGTGTACGTACTACAGGAGCTGAGAAACCAAAGTTTCTGCCA3170.19258224233771756No Hit
CGAGACGATGTGTACGTACTACAGGCCTATCAGAAACGAATGGGGGTGCA3060.18589957777710278No Hit
CGAGACGATGTGTACGTACTACCAGGGAACAACTAGGTCAGAAGTTTGAA3030.18407703289693508No Hit
CGAGACGATGTGTACGTACTACGCGGGATCAGTAGAAACAAGGGTGTTTT2990.18164697305671154No Hit
CGAGACGATGTGTACGTACTACAGGGGGAAAATTGATGGAGTGAAATTGG2980.18103945809665561No Hit
CGAGACGATGTGTACGTACTACGGGGGCAGAAATCATAAAGATGATGGAA2900.1761793384162085No Hit
CGAGACGATGTGTACGTACTACGGGGGGGCAAAGACATAATGGATTCCCA2810.1707117037757055No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGAGTTTTT2690.16342152425503478No Hit
CGAGACGATGTGTACGTACTACCCAGGTGCTGTTTTCATATGTGATGATT2630.15977643449469944No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCAAAAGCAGGAGTTTAAA2620.15916891953464354No Hit
CGAGACGATGTGTACGTACTACCGCAGAAGGCCCTCTTTTTAAACCGTGT2560.1555238297743082No Hit
CGAGACGATGTGTACGTACTACCTCAGCATTGTCTCCGAAGAAATAAGAT2530.1537012848941405No Hit
CGAGACGATGTGTACGTACTACAGGTGCTGTTTTCATATGTGATGATTCT2530.1537012848941405No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCAAAAGCAGGGTGGCAAA2500.15187874001397283No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCGAAAGCAGGGTAGATAA2490.15127122505391696No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCGAAAGCAGGGTGGCAAA2480.15066371009386106No Hit
CGAGACGATGTGTACGTACTACCGGGGATACAATAATATTTGAGGCAAAT2470.15005619513380516No Hit
CGAGACGATGTGTACGTACTACGTGGGATCAATGACCGAAACTTCTGGAG2450.1488411652136934No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCGAAAGCAGGAGTTTAAA2440.1482336502536375No Hit
CGAGACGATGTGTACGTACTACAGGGGGGCAAAGACATAATGGATTCCCA2240.13608335105251967No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGCATTTTT2160.13122323137207254No Hit
CGAGACGATGTGTACGTACTACCGGGGAACAACTAGGTCAGAAGTTTGAA2100.1275781416117372No Hit
CGAGACGATGTGTACGTACTACCCGGGAGCAACTAGGTCAGAAGTTTGAA2090.1269706266516813No Hit
CGAGACGATGTGTACGTACTACCAAAACTACTGTAGACCACATGGCCATA2040.12393305185140185No Hit
CGAGACGATGTGTACGTACTACCGAAGAAGGCCCTCTTTTTAAACCGTGT2020.12271802193129006No Hit
CGAGACGATGTGTACGTACTACAGGGGCAGAAATCATAAAGATGATGGAA1970.1196804471310106No Hit
CGAGACGATGTGTACGTACTACGCGTGTCAGTAGAAACAAGGGTGTTTTT1890.11482032745056348No Hit
CGAGACGATGTGTACGTACTACAAGGGTATTTTTCATTAATTGTCGTACT1890.11482032745056348No Hit
CGAGACGATGTGTACGTACTACGGGGGGACAACTAGGTCAGAAGTTTGAA1830.11117523769022812No Hit
CGAGACGATGTGTACGTACTACAGGGGGGGAACAACTAGGTCAGAAGTTT1810.10996020777011634No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGTACTTTT1790.10874517785000457No Hit
CGAGACGATGTGTACGTACTACAGGGGAAGAACATCAGACATGAGGGCAG1780.10813766288994868No Hit
CGAGACGATGTGTACGTACTACAGGGGAACAACTAGGTCAGAAGTTTGAA1770.10753014792989277No Hit
CGAGACGATGTGTACGTACTACTGGAGGGGTGAGAATGGAAGAAAAACAA1770.10753014792989277No Hit
CGAGACGATGTGTACGTACTACCAGGGAGAAAATTGATGGAGTGAAATTG1720.10449257312961331No Hit
CGAGACGATGTGTACGTACTACCTCCGCATTGTCTCCGAAGAAATAAGAT1710.10388505816955743No Hit
CGAGACGATGTGTACGTACTACCGGGGCAGAAATCATAAAGATGATGGAA1690.10267002824944564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG353.2014214E-1095.284271
CGTGACG156.5297214E-495.284271
ACGTTGT156.609198E-494.9948355
GACGTTG156.609198E-494.9948354
CGTTGTG156.609198E-494.9948356
ACGATGC201.7398439E-594.9948355
CGATGTA156.609198E-494.9948356
GCTGTGT409.094947E-1294.9948357
GTGACGA156.609198E-494.9948352
GTGTGTA254.6044624E-794.994838
CGATGTT254.6044624E-794.994836
ATGTTTA254.6044624E-794.994838
TGTGTAC160400.093.4846349
ACGATGT159750.093.448755
ATGTGTA160900.093.430288
CGAGACG158700.093.423021
CGATGTG160250.093.364666
AGACGAT160000.093.332423
GATGTGT161650.093.084957
GAGACGA159850.093.033732
Produced by FastQC (version 0.11.5)