FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC351CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC351CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6758
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGATACACGTGACGACGCGTGATCAGCAAAAGCAGGGTGACAAAGA560.8286475288546906No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGTAGTTTTTT500.739863865048831No Hit
CGCGCGATACACGTGACGACGCAAAAGCAGGGTGACAAAGACATAATGGA490.7250665877478544No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTT410.6066883693400414No Hit
CGCGCGATACACGTGACGACGCAGAAGCAGGGTGACAAAGACATAATGGA380.5622965374371115No Hit
CGCGCGATACACGTGACGACGCAGCAGAGGCCATGGAGGTTGCTAGTCAG320.4735128736312519No Hit
CGCGCGATACACGTGACGACGCGCGATACGCGTGATCAGTAGAAACAAGG260.38472920982539216No Hit
CGCGCGATACACGTGACGACGCAAGAGCAGGGTGACAAAGACATAATGGA230.34033737792246227No Hit
CGCGCGATACACGTGACGACGCAAAAGCAGGTAGATATTGAAAGATGAGT230.34033737792246227No Hit
CGCGCGATACACGTGACGACGCGTGATCAGCAAAAGCAGGTAGATATTGA230.34033737792246227No Hit
CGCGCGATACACGTGACGACGCCTGCCACTTTCTGCTTGGGTATGAGCAT210.31074282332050907No Hit
CGCGCGATACACGTGACGACGCAGGGTGACAAAGACATAATGGATCCAAA210.31074282332050907No Hit
CGCGCGATACACGTGACGACGCAGGGGGACAAAGACATAATGGATCCAAA200.2959455460195324No Hit
CGCGCGATACACGTGACGACGCAGGGGGGCAAAGACATAATGGATCCAAA180.2663509914175792No Hit
CGCGCGATACACGTGACGACGCGCGATACGCGTGATCAGCAAAAGCAGGG170.25155371411660254No Hit
CGCGCGATACACGTGACGACGCCGGACCCAAAGCCTCTACTCAGTGCGAA150.2219591595146493No Hit
CGCGCGATACACGTGACGACGCCACAAAAAGAAATGCTGCTCGCACTAGT140.20716188221367265No Hit
CGCGCGATACACGTGACGACGCAGGGGAAAATAAAAACAACCAAAATGAA140.20716188221367265No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGGTATTTTTC140.20716188221367265No Hit
CGCGCGATACACGTGACGACGCAGGGGGCAAAGACATAATGGATCCAAAC130.19236460491269608No Hit
CGCGCGATACACGTGACGACGCAGGGGTTTAAAATGAATCCAAATCAGAA120.17756732761171945No Hit
CGCGCGATACACGTGACGACGCAGGCCCTCTTTGTATCAGAATGGACCAG110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCGGAGGAAGAAAATTTTGTACGAGGCCAG110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCAGAAGCAGGTAGATATTGAAAGATGAGT110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCAAAAGCAGGGGTTTAAAATGAATCCAAA110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCGTGATCAGCAAAAGCAGGGGTTTAAAAT110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCGGGGGGACAAAGACATAATGGATCCAAA110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCAACAGCAGGGTGACAAAGACATAATGGA110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCAGGGGGCCAAAGACATAATGGATCCAAA110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCGGGCGGGGAAAGATCCTAAGAAAACTGG110.16277005031074282No Hit
CGCGCGATACACGTGACGACGCAGGTACAGAGGCCATGGTCATTTTAAGT100.1479727730097662No Hit
CGCGCGATACACGTGACGACGCAGTAGAAACAAGGGTGTTTTTTATTATT100.1479727730097662No Hit
CGCGCGATACACGTGACGACGCGGGGGACAAAGACATAATGGATCCAAAC90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCAGCAAAAGCAGGGTGACAAAGACATAAT90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCCGGCCAAAAATCACACTGAAGTTCGCTT90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCTTGGGTATGAGCATGGACCAGTCCCTTG90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGAGTTTTTTG90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCAGAAGCAGGGGTTTAAAATGAATCCAAA90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCATAAGCAGGGTGACAAAGACATAATGGA90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCAGGCCCCCTCAAAGCCGAGATCGCACAG90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCCGGGGGACAAAGACATAATGGATCCAAA90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTC90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCATGATCAGCAAAAGCAGGGTGACAAAGA90.1331754957087896No Hit
CGCGCGATACACGTGACGACGCAGTAGAAACAAGGTAGTTTTTTACTCCA80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCATAAGCAGGGGTTTAAAATGAATCCAAA80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCCACAACAAGAAATGCTGCTCGCACTAGT80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCAGAGGCCATGGCCTCTGCTGCTTGCTCA80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCGTGATCGGAAGAGCACACGTCTGAACTC80.11837821840781297Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGCGCGATACACGTGACGACGCAGGGGACAAAGACATAATGGATCCAAAC80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCGAAAGGAGAAATAAATACCTGGAAGAAC80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCAACAGGGGAAAATAAAAACAACCAAAAT80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCCACAGAAAGAAATGCTGCTCGCACTAGT80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCAGCAGCGGCCATGGAGGTTGCTAGTCAG80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCACGCCACAAAAAGAAATGCTGCTCGCAC80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCCCGCGCGATACGCGTGATCAGTAGAAAC80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCAAAAAGAAATGCTGCTCGCACTAGTCCA80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCAAAAGCAGGGTAGATAATCACTCACTGA80.11837821840781297No Hit
CGCGCGATACACGTGACGACGCAAAGCCTCTACTCAGTGCGAAAGCATAC70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCAAGAGCAGGTAGATATTGAAAGATGAGT70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCGGAAGGAGAAATAAATACCTGGAAGAAC70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCAAAGAGGGAATATAATCTCAATATGGAT70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCAGGGGACGAAGACATAATGGATCCAAAC70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCAGGGGGAGAAAGACATAATGGATCCAAA70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCAGCAGCGGCCATGGCCTCTGCTGCTTGC70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCCAGCTCTATGCTGACAAAATGACCATCG70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCGGCGGGGAAAGATCCTAAGAAAACTGGA70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGTCGTTTTTA70.10358094110683633No Hit
CGCGCGATACACGTGACGACGCGAGATCGGAAGAGCACACGTCTGAACTC70.10358094110683633Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG6650.095.09
CGATACA6650.095.05
GATACAC6650.095.06
TACACGT6600.095.08
ATACACG6650.095.07
GCGATAC7000.090.254
CGCGATA7000.090.253
CGCGCGA7150.089.685311
GCGCGAT7100.089.647892
CGACGCA3050.047.50000416-17
ACGCCAC252.6802056E-547.518-19
CACGTGA6700.047.510-11
ACGCAGC509.094947E-1247.518-19
ACGCAGA252.6802056E-547.518-19
ACGCACA301.3848858E-647.518-19
GGACAAA252.6802056E-547.526-27
GACGCTG252.6802056E-547.518-19
GCAGCAG252.6802056E-547.520-21
GACGCGC252.6802056E-547.518-19
GACGCGA451.9281288E-1047.518-19
Produced by FastQC (version 0.11.5)