FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC348CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC348CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145847
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGTAGTTTTTTACTCTA12850.8810602892071828No Hit
CGTCGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC10240.7021056312437006No Hit
CGTCGAGACACTACGCAGCGTCAGGAGAAACAAGGTAGTTTTTTACTCTA8050.5519482745616983No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGTCGTTTTTAAATTAT7300.5005245222733412No Hit
CGTCGAGACACTACGCAGCGTCCCGAAGATGGAGAGGGCAGCTGCAATCC6740.4621281205647014No Hit
CGTCGAGACACTACGCAGCGTCAGGCCCCCTCAAAGCCGAGATCGCACAG5470.3750505666897502No Hit
CGTCGAGACACTACGCAGCGTCATCAGCAAAAGCAGGTAGATATTGAAAG5460.37436491665923877No Hit
CGTCGAGACACTACGCAGCGTCAAAAGCAGGTAGATATTGAAAGATGAGT5240.35928061598798744No Hit
CGTCGAGACACTACGCAGCGTCATCAGCGAAAGCAGGTAGATATTGAAAG5210.3572236658964531No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGTACTTTTTTGGACAG4390.3010003633945162No Hit
CGTCGAGACACTACGCAGCGTCAGACCAGTGCTAGAGCTAGGATGAGTCC4380.3003147133640048No Hit
CGTCGAGACACTACGCAGCGTCATACTCCATATTTTTGGCTGGACCAATT4230.2900299629063333No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGCATTTTTTCATGAAG3670.2516335611976935No Hit
CGTCGAGACACTACGCAGCGTCAGAAGCTTGAAGAAGTAAGATGGCTGAT3490.2392918606484878No Hit
CGTCGAGACACTACGCAGCGTCATCAGCGAAAGCAGGGTGGCAAAGACAT3270.22420755997723643No Hit
CGTCGAGACACTACGCAGCGTCAGCAAAAGCAGGTAGATATTGAAAGATG3260.223521909946725No Hit
CGTCGAGACACTACGCAGCGTCAGGAGAAACAAGGTCGTTTTTAAATTAT3220.2207793098246793No Hit
CGTCGAGACACTACGCAGCGTCATCAGCAAAAGCAGGGTGGCAAAGACAT3140.21529410958058787No Hit
CGTCGAGACACTACGCAGCGTCAGGCAAGACTTGTGAGCAACTGATCCTC2960.2029524090313822No Hit
CGTCGAGACACTACGCAGCGTCAGGAGAAACAAGGTACTTTTTTGGACAG2890.1981528588178022No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGGTGTTTTTTATCACT2850.1954102586957565No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGGTATTTTTCATTAAT2810.19266765857371082No Hit
CGTCGAGACACTACGCAGCGTCAACAACAAGAGGATCACTTGAATCGTTG2760.18923940842115367No Hit
CGTCGAGACACTACGCAGCGTCAGAAGAAACAAGGTAGTTTTTTACTCTA2710.18581115826859654No Hit
CGTCGAGACACTACGCAGCGTCAGGCAAACCATTTGAATGGATGTCAATC2660.1823829081160394No Hit
CGTCGAGACACTACGCAGCGTCAAAAGCAGGGTGGCAAAGACATAATGGA2460.1686699075058109No Hit
CGTCGAGACACTACGCAGCGTCTGAAACGAAAACGTTCTAATCTCTTGTT2380.16318470726171946No Hit
CGTCGAGACACTACGCAGCGTCAGGGGGGCAAAGACATAATGGATTCCCA2360.16181340720069662No Hit
CGTCGAGACACTACGCAGCGTCAGAAACGAAAACGTTCTAATCTCTTGTT2330.15975645710916234No Hit
CGTCGAGACACTACGCAGCGTCAGCAGAAGCAGGTAGATATTGAAAGATG2300.15769950701762805No Hit
CGTCGAGACACTACGCAGCGTCAAACCATTTGAATGGATGTCAATCCGAC2290.15701385698711662No Hit
CGTCGAGACACTACGCAGCGTCTGGCCAAGACCAGTTTGCAGTATCACTA2200.1508430067125138No Hit
CGTCGAGACACTACGCAGCGTCAAAGCAGGAAGATATTGAAAGATGAGTC2090.1433008563768881No Hit
CGTCGAGACACTACGCAGCGTCAGGCCATGGAGGTTGCTAGTCAGGCCAG2080.14261520634637667No Hit
CGTCGAGACACTACGCAGCGTCCCCAGCTATCTCAATGCATGTGTTAGGA1980.13575870604126242No Hit
CGTCGAGACACTACGCAGCGTCATCACCATTGTTGGCTTGGCGCCAAATC1960.13438740598023954No Hit
CGTCGAGACACTACGCAGCGTCAGCAAAAGCAGGTACTGATTCAAAATGG1920.13164480585819385No Hit
CGTCGAGACACTACGCAGCGTCATCAGCGAAAGCAGGTACTGATTCAAAA1920.13164480585819385No Hit
CGTCGAGACACTACGCAGCGTCATCAGCAAAAGCAGGTACTGATTCAAAA1910.13095915582768244No Hit
CGTCGAGACACTACGCAGCGTCAACAGCAAGAGGATCACTTGAATCGTTG1900.130273505797171No Hit
CGTCGAGACACTACGCAGCGTCAGGGGGGGAAAGACATAATGGATTCCCA1870.12821655570563673No Hit
CGTCGAGACACTACGCAGCGTCAGCAAAAGCAGGGTGGCAAAGACATAAT1840.12615960561410247No Hit
CGTCGAGACACTACGCAGCGTCCATGGCCTCAGCTGCTTGTTCGCTCGAT1770.12136005540052247No Hit
CGTCGAGACACTACGCAGCGTCAGCAGAAGCAGGGTGGCAAAGACATAAT1750.1199887553394996No Hit
CGTCGAGACACTACGCAGCGTCAAAAGCAGGTACTGATTCAAAATGGAAG1720.11793180524796534No Hit
CGTCGAGACACTACGCAGCGTCATCAGCAAAAGCAGGTCAATTATATTCA1700.11656050518694248No Hit
CGTCGAGACACTACGCAGCGTCGGGGGGCAAAGACATAATGGATTCCCAC1630.1117609549733625No Hit
CGTCGAGACACTACGCAGCGTCCTGCATACTTTCCCAATGGAACCTTCTT1610.11038965491233965No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGAGTTTTTTTCAACGA1600.10970400488182822No Hit
CGTCGAGACACTACGCAGCGTCATCAGCAAAAGCAGGCAAACCATTTGAA1580.10833270482080537No Hit
CGTCGAGACACTACGCAGCGTCAGGAGAAACAAGGCATTTTTTCATGAAG1540.10559010469875967No Hit
CGTCGAGACACTACGCAGCGTCAGCAGAAGCAGGTACTGATTCAAAATGG1500.10284750457671395No Hit
CGTCGAGACACTACGCAGCGTCAGGGGGCAAAGACATAATGGATTCCCAC1500.10284750457671395No Hit
CGTCGAGACACTACGCAGCGTCAGGCCAAGACCAGTTTGCAGTATCACTA1470.10079055448517968No Hit
CGTCGAGACACTACGCAGCGTCAGGACCCCTCAAAGCCGAGATCGCACAG1470.10079055448517968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAG301.2070814E-895.193821
CGTGGAG201.721187E-595.193811
CGTTGAG201.721187E-595.193811
GTTGAGA201.7388915E-594.997952
CACTAGG409.094947E-1294.997959
CGCTACG156.6068093E-494.997959
ACAATAC156.6068093E-494.997958
ACGCTAC156.6068093E-494.997958
GACGCTA156.6068093E-494.997957
ACGAGAC201.7388915E-594.997953
ACACGAC254.6009154E-794.997948
CACGACG254.6009154E-794.997949
AGACACG254.6009154E-794.997946
CACTACG145350.093.984899
GAGACAC145400.093.919915
GACACTA145550.093.8883747
AGACACT145400.093.887246
CGAGACA145150.093.85264
TCGAGAC145050.093.819073
ACACTAC145600.093.595158
Produced by FastQC (version 0.11.5)