FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC346CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC346CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684881
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTATATACTCTAGCGAGTGATTAGCAAAAGCAGGTAGATATTGAAAG53240.7773613226239303No Hit
CGTGTATATACTCTAGCGAGTGGCCGAGACAAGACAAATCCTAAAATCCC47440.6926750778602414No Hit
CGTGTATATACTCTAGCGAGTGATTAGCGAAAGCAGGTAGATATTGAAAG45720.6675612259648026No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCAAAAGCAGGTAGATATTGAAAG45120.6588005799547659No Hit
CGTGTATATACTCTAGCGAGTGAACAGCAAAAGCAGGTAGATATTGAAAG44420.6485798262763895No Hit
CGTGTATATACTCTAGCGAGTGAACAGCGAAAGCAGGTAGATATTGAAAG42760.6243420389819545No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTGAAAG42680.6231739528472829No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCGAAAGCAGGTAGATATTGAAAG38260.5586371939066787No Hit
CGTGTATATACTCTAGCGAGTGATCAGCGAAAGCAGGTAGATATTGAAAG36210.5287049867057197No Hit
CGTGTATATACTCTAGCGAGTGGCAAGGACAAGACAAATCCTAAAATCCC33850.4942464457329083No Hit
CGTGTATATACTCTAGCGAGTGGCCAAGACAAGACAAATCCTAAAATCCC32750.47818526138117423No Hit
CGTGTATATACTCTAGCGAGTGACCAGCAAAAGCAGGTAGATATTGAAAG29640.4327759128958169No Hit
CGTGTATATACTCTAGCGAGTGCAGCGAAAGCAGGTAGATATTGAAAGAT28290.4130644593732342No Hit
CGTGTATATACTCTAGCGAGTGACCAGCGAAAGCAGGTAGATATTGAAAG27600.4029897164616919No Hit
CGTGTATATACTCTAGCGAGTGATAAGCAAAAGCAGGTAGATATTGAAAG26240.38313225217227515No Hit
CGTGTATATACTCTAGCGAGTGATAAGCGAAAGCAGGTAGATATTGAAAG24230.35378408803865197No Hit
CGTGTATATACTCTAGCGAGTGATGAGCAAAAGCAGGTAGATATTGAAAG22030.3216617193351838No Hit
CGTGTATATACTCTAGCGAGTGCTCAGCAAAAGCAGGTAGATATTGAAAG21660.31625932096232773No Hit
CGTGTATATACTCTAGCGAGTGGCGCAGACAAGACAAATCCTAAAATCCC21210.30968883645480016No Hit
CGTGTATATACTCTAGCGAGTGATGAGCGAAAGCAGGTAGATATTGAAAG20150.29421169517040185No Hit
CGTGTATATACTCTAGCGAGTGGCACAGACAAGACAAATCCTAAAATCCC20020.29231355520156055No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCAAATGCAACAACAAGAGGATC19540.2853050383935311No Hit
CGTGTATATACTCTAGCGAGTGCGGAGGCCATGGTCATTTTAAGTGCCTC18000.2628193803011034No Hit
CGTGTATATACTCTAGCGAGTGCTCAGCGAAAGCAGGTAGATATTGAAAG16770.244860055980528No Hit
CGTGTATATACTCTAGCGAGTGGCGACGACAAGACAAATCCTAAAATCCC16630.24281590524485275No Hit
CGTGTATATACTCTAGCGAGTGGCAAAGGCAAGACAAATCCTAAAATCCC15830.23113504389813705No Hit
CGTGTATATACTCTAGCGAGTGGCCAGGACAAGACAAATCCTAAAATCCC15510.22646269935945074No Hit
CGTGTATATACTCTAGCGAGTGGCCCAGACAAGACAAATCCTAAAATCCC15480.22602466705894894No Hit
CGTGTATATACTCTAGCGAGTGCAGCAAAAGCAGGTAGATATTGAAAGAT15170.2214983332870966No Hit
CGTGTATATACTCTAGCGAGTGGGGGTGCAGATGCAACGATTCAAGTGAT14920.21784806411624794No Hit
CGTGTATATACTCTAGCGAGTGGGGGGCTATAAGAAGCATTAAGCAAAAC14480.2114235903755543No Hit
CGTGTATATACTCTAGCGAGTGGGGGGCTATATGAAGCAATTGAGGAGTG14120.2061672027695322No Hit
CGTGTATATACTCTAGCGAGTGATCCGCAAAAGCAGGTAGATATTGAAAG13820.20178687976451382No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCAAAAGCAGGGTGGCAAAGACAT13320.1944863414228165No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCGAAAGACAAGACAAATCCTAA13270.19375628758864677No Hit
CGTGTATATACTCTAGCGAGTGATCCGCGAAAGCAGGTAGATATTGAAAG13020.19010601841779812No Hit
CGTGTATATACTCTAGCGAGTGGCGAAGACAAGACAAATCCTAAAATCCC12650.18470362004494212No Hit
CGTGTATATACTCTAGCGAGTGAAAGCAGGAAGATATTGAAAGATGAGTC12540.1830975016097687No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCCAAAGACAAGACAAATCCTAA12520.1828054800761008No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCGAAGACATAATGGATTCCCAC11680.1705405756620493No Hit
CGTGTATATACTCTAGCGAGTGATCAACGAAAGCAGGTAGATATTGAAAG11580.16908046799370985No Hit
CGTGTATATACTCTAGCGAGTGATCAACAAAAGCAGGTAGATATTGAAAG11350.1657222203565291No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCAAAAGCAGGTACTGATTCAAAA10970.16017381121683913No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCAAAAGACAAGACAAATCCTAA10900.1591517358490015No Hit
CGTGTATATACTCTAGCGAGTGGCAACGACAAGACAAATCCTAAAATCCC10640.1553554559113189No Hit
CGTGTATATACTCTAGCGAGTGACAAGCAGCTGAGGCCATGGAGGTTGCT10470.1528732728751418No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAGAAACAAGGTAGTTTTTTACT10290.15024507907213078No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCAAATGCAACGATTCAAGTGAT10220.14922300370429317No Hit
CGTGTATATACTCTAGCGAGTGCACAATCCCAATTATACTTGCGGCAACA10080.1471788529686179No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCGAAAGCAGGTACTGATTCAAAA10060.14688683143495002No Hit
CGTGTATATACTCTAGCGAGTGATTAGCAAAAGCAGGGTGGCAAAGACAT9970.1455727345334445No Hit
CGTGTATATACTCTAGCGAGTGATCAGCGAAAGCAGGGTGGCAAAGACAT9850.14382060533143715No Hit
CGTGTATATACTCTAGCGAGTGATTAGCAAAAGCAGGTACTGATTCAAAA9830.14352858379776925No Hit
CGTGTATATACTCTAGCGAGTGATCATCAAAAGCAGGTAGATATTGAAAG9730.14206847612942977No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGGTGGCAAAGACAT9550.13944028232641875No Hit
CGTGTATATACTCTAGCGAGTGGGTGGCAAAGACATAATGGATTCCCACA9500.138710228492249No Hit
CGTGTATATACTCTAGCGAGTGACCAGCGAAAGCAGGTCAATTATATTCA9440.13783416389124534No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCGAATGCAACAACAAGAGGATC9280.1354979916219022No Hit
CGTGTATATACTCTAGCGAGTGAACAGCAAAAGCAGGTACTGATTCAAAA9180.13403788395356273No Hit
CGTGTATATACTCTAGCGAGTGATCATCGAAAGCAGGTAGATATTGAAAG9040.1319937332178875No Hit
CGTGTATATACTCTAGCGAGTGACCAGCAAAAGCAGGTACTGATTCAAAA9030.13184772245105353No Hit
CGTGTATATACTCTAGCGAGTGACCAGCGAAAGCAGGTACTGATTCAAAA8850.1292195286480425No Hit
CGTGTATATACTCTAGCGAGTGAACAGCAAAAGCAGGGTGGCAAAGACAT8840.12907351788120858No Hit
CGTGTATATACTCTAGCGAGTGGGCGATCAATAATCCACAATATCAAGTG8800.12848947481387277No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGGCAAAGACATAATGGATTCCCA8730.12746739944603516No Hit
CGTGTATATACTCTAGCGAGTGAACAGCGAAAGCAGGTACTGATTCAAAA8720.1273213886792012No Hit
CGTGTATATACTCTAGCGAGTGATTAGCGAAAGCAGGTACTGATTCAAAA8620.12586128101086175No Hit
CGTGTATATACTCTAGCGAGTGGCCGGGACAAGACAAATCCTAAAATCCC8620.12586128101086175No Hit
CGTGTATATACTCTAGCGAGTGCGGCAACGATTCAAGTGATCCTCTTGTT8590.1254232487103599No Hit
CGTGTATATACTCTAGCGAGTGGGGCAGGGAATGAGTCCCAATGGCTCTC8480.1238171302751865No Hit
CGTGTATATACTCTAGCGAGTGGCGGCGACAAGACAAATCCTAAAATCCC8450.12337909797468466No Hit
CGTGTATATACTCTAGCGAGTGCAGCGAAAGCAGGTACTGATTCAAAATG8430.12308707644101675No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCGAAAGCAGGGTGGCAAAGACAT8370.12221101184001308No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGGGAACTCGTCCTTTATGACAAA8340.12177297953951124No Hit
CGTGTATATACTCTAGCGAGTGGCGCCGACAAGACAAATCCTAAAATCCC8320.12148095800584334No Hit
CGTGTATATACTCTAGCGAGTGGAGGCGGCAACCTGTGAACAGATTGCCG8200.11972882880383601No Hit
CGTGTATATACTCTAGCGAGTGGGGTGCAGATGCAACGATTCAAGTGATC8120.11856074266916443No Hit
CGTGTATATACTCTAGCGAGTGGCAGAGACAAGACAAATCCTAAAATCCC8060.11768467806816076No Hit
CGTGTATATACTCTAGCGAGTGAGGGGGGCAAAGACATAATGGATTCCCA7980.11651659193348918No Hit
CGTGTATATACTCTAGCGAGTGATCAGCGAAAGCAGGTACTGATTCAAAA7900.1153485057988176No Hit
CGTGTATATACTCTAGCGAGTGGCGGAGACAAGACAAATCCTAAAATCCC7830.11432643043097998No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTACTGATTCAAAA7790.11374238736364418No Hit
CGTGTATATACTCTAGCGAGTGATTAGCGAAAGCAGGGTGGCAAAGACAT7010.10235354755059638No Hit
CGTGTATATACTCTAGCGAGTGAACAGCGAAAGCAGGGTGGCAAAGACAT7010.10235354755059638No Hit
CGTGTATATACTCTAGCGAGTGAGAAGCGAAAGCAGGTAGATATTGAAAG6850.10001737528125323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAA409.094947E-1295.1831051
CGTGTAG550.095.18311
GTACATA301.2316377E-894.9954764
GTATCTA254.6199602E-794.9954764
CGTGTAT672200.094.93531
TATACTC672500.094.7341467
ATACTCT672700.094.621258
ATATACT673600.094.579446
GTATATA674750.094.523844
GTGTATA676750.094.455052
TATATAC674250.094.4248665
TGTATAT676350.094.398553
TACTCTA686800.092.651029
CGTGTGT1400.091.78371
GTATGTA950.089.995714
ATATACG850.089.40756
ATACGCT800.089.058268
TATACGC800.089.058267
GCATATA800.089.058264
CGCGTAT600.087.251181
Produced by FastQC (version 0.11.5)