FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC345CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC345CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256936
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCTA44621.7366192359186725No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT36551.4225332378491142No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTATTTTTCATTAAT32501.2649064358439455No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGTAGTTTTTTACTCTA16960.6600865585204098No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTCGTTTTTAAATTAT15640.6087118971261326No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGTATTTTTCATTAAT12360.48105364760095903No Hit
CGAGACGTAGATGACGAGTATCGGGGCGGGAACAACTAGGTCAGAAGTTT11840.4608151446274559No Hit
CGAGACGTAGATGACGAGTATCCAGGCGGGAACAACTAGGTCAGAAGTTT11260.43824142977239466No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTCCTTATA10670.4152785129370738No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGTGTTTTTTATCACT9840.3829747485755207No Hit
CGAGACGTAGATGACGAGTATCCGGGCGGGAACAACTAGGTCAGAAGTTT9770.380250334713703No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTACTTTTTTGGACAG8220.3199240277734533No Hit
CGAGACGTAGATGACGAGTATCCCGCGCTGGGATGTTCCTCTAGATATTT7970.31019397826696143No Hit
CGAGACGTAGATGACGAGTATCAGCAGAAGCAGGTAGATATTGAAAGATG7920.30824796836566304No Hit
CGAGACGTAGATGACGAGTATCAGCAGAAGCAGGGTGGCAAAGACATAAT6880.2677709624186568No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGTCGTTTTTAAATTAT6060.23585640003736338No Hit
CGAGACGTAGATGACGAGTATCGAGGCGGGAACAACTAGGTCAGAAGTTT5950.23157517825450696No Hit
CGAGACGTAGATGACGAGTATCAGGGCGGGAACAACTAGGTCAGAAGTTT4980.19382258616931844No Hit
CGAGACGTAGATGACGAGTATCGAGATCGGAAGAGCACACGTCTGAACTC4740.18448173864308623Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAGACGTAGATGACGAGTATCAGCAAAAGCAGGTAGATATTGAAAGATG4700.18292493072204752No Hit
CGAGACGTAGATGACGAGTATCGGGGGCAGAAATCATAAAGATGATGGAA4580.1782545069589314No Hit
CGAGACGTAGATGACGAGTATCCGTAGAAGGCCCTCTTTTTAAACCGTGT4580.1782545069589314No Hit
CGAGACGTAGATGACGAGTATCAAGGCGGGAACAACTAGGTCAGAAGTTT4530.17630849705763302No Hit
CGAGACGTAGATGACGAGTATCAGGAGAAACAAGGTAGTTTTTTACTCTA4520.17591929507737336No Hit
CGAGACGTAGATGACGAGTATCAGCAAAAGCAGGGTGGCAAAGACATAAT4400.17124887131425723No Hit
CGAGACGTAGATGACGAGTATCTACGCGTGATCAGTAGAAACAAGGGTGT4190.16307562972880407No Hit
CGAGACGTAGATGACGAGTATCAGAAACGAATGGGGGTGCAGATGCAACG4130.16074041784724602No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGTGTTTTTCCTTATA4080.15879440794594762No Hit
CGAGACGTAGATGACGAGTATCCGAAGAATCAAAGTTAAACAGGGAGAAA4070.15840520596568797No Hit
CGAGACGTAGATGACGAGTATCCAGCCATTTGCTCCATAGCCTTAGCTGT4030.15684839804464926No Hit
CGAGACGTAGATGACGAGTATCGCGGGGACAACTAGGTCAGAAGTTTGAA3820.14867515645919605No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGTACTTTTTTGGACAG3750.14595074259737834No Hit
CGAGACGTAGATGACGAGTATCGCGGCGGGAACAACTAGGTCAGAAGTTT3550.13816670299218484No Hit
CGAGACGTAGATGACGAGTATCAGCAGAAGCAGGGTAGATAATCACTCAC3520.1369990970514058No Hit
CGAGACGTAGATGACGAGTATCTTGGGGGGCTATATGAAGCAATTGAGGA3480.1354422891303671No Hit
CGAGACGTAGATGACGAGTATCCAGGGAACAACTAGGTCAGAAGTTTGAA3370.13116106734751065No Hit
CGAGACGTAGATGACGAGTATCAGGGGGGCAAAGACATAATGGATTCCCA3340.12999346140673163No Hit
CGAGACGTAGATGACGAGTATCACAGGCAAATGGTAACAACAACCAATCC3330.12960425942647194No Hit
CGAGACGTAGATGACGAGTATCGAAAGGGGAAAAAGCTAATGTGCTAATT3320.12921505744621228No Hit
CGAGACGTAGATGACGAGTATCGAGGGCAGAAGTTTGAAGAAGTAAGATG3310.1288258554659526No Hit
CGAGACGTAGATGACGAGTATCAAGTGCACAATCCCAATTATACTTGCGG3270.12726904754491392No Hit
CGAGACGTAGATGACGAGTATCAGCCGAGTGATGGTATCACCCCTGGCTG3250.12649064358439457No Hit
CGAGACGTAGATGACGAGTATCCAACACAAGTAGCTGTTTCGATGTTCAG3220.12532303764361555No Hit
CGAGACGTAGATGACGAGTATCGGGGGGGCAATATGAAGCAATTGAGGAG3200.12454463368309619No Hit
CGAGACGTAGATGACGAGTATCAGGAGAAACAAGGGTATTTTTCATTAAT3200.12454463368309619No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGAGTTTTTTTCAACGA3140.12220942180153813No Hit
CGAGACGTAGATGACGAGTATCCAGCGCGGGGAAAGATCCTAAGAAAACT3090.12026341190023976No Hit
CGAGACGTAGATGACGAGTATCGCAGAGGGAAGAACATCAGACATGAGGG3070.1194850079397204No Hit
CGAGACGTAGATGACGAGTATCGGGGGGGGAACAACTAGGTCAGAAGTTT3040.11831740199894138No Hit
CGAGACGTAGATGACGAGTATCCGGGGCAGAAATCATAAAGATGATGGAA3010.11714979605816234No Hit
CGAGACGTAGATGACGAGTATCGAGGGCGGAAATCATAAAGATGATGGAA2990.116371392097643No Hit
CGAGACGTAGATGACGAGTATCAGGAGAAACAAGGGTGTTTTTTATCACT2950.11481458417660428No Hit
CGAGACGTAGATGACGAGTATCCCGGGAACAACTAGGTCAGAAGTTTGAA2950.11481458417660428No Hit
CGAGACGTAGATGACGAGTATCCAGAGTCTATGAGGGAAGAATATCGAGA2810.10936575645296882No Hit
CGAGACGTAGATGACGAGTATCGGGGGGGCAAAGACATAATGGATTCCCA2770.10780894853193013No Hit
CGAGACGTAGATGACGAGTATCGGTCGAGTTGTTAGCATGGTAGCCTATA2770.10780894853193013No Hit
CGAGACGTAGATGACGAGTATCGAGGGCAGAAATCATAAAGATGATGGAA2760.10741974655167047No Hit
CGAGACGTAGATGACGAGTATCAAAAGCAGAGAGCACCATTCTCTCTATT2750.10703054457141079No Hit
CGAGACGTAGATGACGAGTATCCAGGGAGAAAATTGATGGAGTGAAATTG2740.10664134259115111No Hit
CGAGACGTAGATGACGAGTATCGGGGCAGAAATCATAAAGATGATGGAAA2700.1050845346701124No Hit
CGAGACGTAGATGACGAGTATCGGGGCCGCAAGTATAATTGGGATTGTGC2690.10469533268985273No Hit
CGAGACGTAGATGACGAGTATCAGCAAAAGCAGGGTAGATAATCACTCAC2680.10430613070959306No Hit
CGAGACGTAGATGACGAGTATCGCCGGTGAATGAGAAAATGTGAATGTGT2640.10274932278855435No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGCATTTTTTCATGAAG2640.10274932278855435No Hit
CGAGACGTAGATGACGAGTATCCACAGCGGAAGTTTCGTCCAACATCCTG2620.101970918828035No Hit
CGAGACGTAGATGACGAGTATCCGAAGAAATAAGATCCTTCATTACTCAT2590.10080331288725597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG201.7313943E-595.1085361
AACGTAG500.094.9974754
TAGACGT500.094.9974752
TACGTAG201.7414719E-594.9974754
GACGTAT301.2283635E-894.997474
GTAGGTG600.094.997477
ATATGAC301.2283635E-894.997479
CGTAGGT600.094.997476
TAGATCA156.612728E-494.997478
TAGATGA256450.092.737828
CGTAGAT254750.092.7227556
ACGTAGA254700.092.703655
AGATGAC255300.092.671849
GAGACGT252600.092.6281742
GACGTAG255100.092.614154
AGACGTA254300.092.606653
CGAGACG253300.092.442641
GTAGATG258750.092.097067
GGCGTAG850.089.409384
GACGTGG800.089.0601354
Produced by FastQC (version 0.11.5)