FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC337CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC337CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428868
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGAGTAGTACAGTGTCTGAAACGAAAACGTTCTAATCTCTTGTTCCA32550.7589747894457035No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGCGAAGACATAATGGATTCCCAC30970.722133616870459No Hit
CGTAGAGTAGTACAGTGTCTGAACTCCAGTCACACAGTGATCTCGTATGC23560.5493531809321283TruSeq Adapter, Index 5 (100% over 34bp)
CGTAGAGTAGTACAGTGTCTGAAGAAGAATCCGATGAGGCACTTAAAATG21150.4931587341559641No Hit
CGTAGAGTAGTACAGTGTCTGACCTAGTTGTTCCCGCCATTTTCCGTTTC17890.41714466922223153No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGCAAAGACATAATGGATTCCCAC16770.39102940764990624No Hit
CGTAGAGTAGTACAGTGTCTGACAGAGCAGTCAAACTTTATCGAAAGCTT15310.3569862988145537No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGGCAAAGACATAATGGATTCCCA14550.3392652284619044No Hit
CGTAGAGTAGTACAGTGTCTGATAGGCCTGTAAATTTTCAAGGAGATCAT13910.32434222184914707No Hit
CGTAGAGTAGTACAGTGTCTGAAGGGGGGCAAAGACATAATGGATTCCCA11400.2658160552897395No Hit
CGTAGAGTAGTACAGTGTCTGACTAGCAACCTCCATGGCCTCAGCTGCTT10870.25345794043854986No Hit
CGTAGAGTAGTACAGTGTCTGAGCAGGGTGGCAAAGACATAATGGATTCC10280.2397007937174142No Hit
CGTAGAGTAGTACAGTGTCTGAAACCGAGGTCGAAACGTACGTTCTCTCT9830.22920805469281927No Hit
CGTAGAGTAGTACAGTGTCTGAAGGCCCTCTTTTTAAACCGTGTTTAAAG9300.2168499398416296No Hit
CGTAGAGTAGTACAGTGTCTGAAAACCCTTTTACTCCGTTTGCTCCATCA9280.21638359588498093No Hit
CGTAGAGTAGTACAGTGTCTGACCGAGGTCGAAACGTACGTTCTCTCTAT9190.21428504808006194No Hit
CGTAGAGTAGTACAGTGTCTGAAAAAGCAGGTAGATATTGAAAGATGAGT8770.20449182499043994No Hit
CGTAGAGTAGTACAGTGTCTGAGGTCTTCAATGGTGGAACAGATCTTCAT8770.20449182499043994No Hit
CGTAGAGTAGTACAGTGTCTGAAGGGGGCAAAGACATAATGGATTCCCAC8280.19306639805254763No Hit
CGTAGAGTAGTACAGTGTCTGAAAAAGCAATGAAAGAGTATGGAGAGGAC7760.18094145517968233No Hit
CGTAGAGTAGTACAGTGTCTGAAAAAGCAGGGTGGCAAAGACATAATGGA7600.177210703526493No Hit
CGTAGAGTAGTACAGTGTCTGACCCAGCTATCTCAATGCATGTGTTAGGA7230.16858334032849268No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGCGGGAACAACTAGGTCAGAAGTTT7020.16368672878368168No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGCCAAGACATAATGGATTCCCAC6990.1629872128487087No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGCCTGACGGGATGATAGAGAGAAC6810.1587901172388707No Hit
CGTAGAGTAGTACAGTGTCTGACGGGGGGCAAAGACATAATGGATTCCCA6620.15435984965070837No Hit
CGTAGAGTAGTACAGTGTCTGACGGGGTACAAACCATTCTTCCCCGTCAG6110.1424680787561674No Hit
CGTAGAGTAGTACAGTGTCTGACGCGTGATCAGTAGAAACAAGGTAGTTT6100.14223490677784306No Hit
CGTAGAGTAGTACAGTGTCTGACCGAGAGTGCTGCCTCTTCCCTTTAGAG6000.13990318699459975No Hit
CGTAGAGTAGTACAGTGTCTGAGGTCGAAACGTACGTTCTCTCTATCATC5980.13943684303795106No Hit
CGTAGAGTAGTACAGTGTCTGACAAAATGATCATCGTCAGCATCCACAGC5890.13733829523303206No Hit
CGTAGAGTAGTACAGTGTCTGAATGAGCGGGTCATTATGGTGAAACATGA5670.13220851170989675No Hit
CGTAGAGTAGTACAGTGTCTGAGGGAGGCAAAGACATAATGGATTCCCAC5620.13104265181827507No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGAGGCAAAGACATAATGGATTCCCA5610.13080947983995075No Hit
CGTAGAGTAGTACAGTGTCTGAGGGCGGCAAAGACATAATGGATTCCCAC5600.13057630786162644No Hit
CGTAGAGTAGTACAGTGTCTGATGTTCTTCCCTCTGTATTCCCAGAGAAT5010.11681916114049079No Hit
CGTAGAGTAGTACAGTGTCTGAAGGGAGGCAAAGACATAATGGATTCCCA4990.11635281718384212No Hit
CGTAGAGTAGTACAGTGTCTGAAGAAACAAGGTAGTTTTTTACTCTAGCT4950.11542012927054479No Hit
CGTAGAGTAGTACAGTGTCTGATTGAGGAAGTGAGGCATAAATGTTATTT4900.11425426937892312No Hit
CGTAGAGTAGTACAGTGTCTGATGGCCATCCGAATTCTTTTGGTCGCTGT4870.11355475344395011No Hit
CGTAGAGTAGTACAGTGTCTGAAAGCCCTTAGTAATGTCAGATTCTCCAA4680.1091244858557878No Hit
CGTAGAGTAGTACAGTGTCTGAAAGAGAATCCGATGAGGCACTTAAAATG4670.10889131387746345No Hit
CGTAGAGTAGTACAGTGTCTGACGCGTGATCAGTAGAAACAAGGTACTTT4590.1070259380508688No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGGAAAGACATAATGGATTCCCAC4490.10469421826762547No Hit
CGTAGAGTAGTACAGTGTCTGACTTAGCCAACAGTGTCCTGCATACTTTC4480.10446104628930113No Hit
CGTAGAGTAGTACAGTGTCTGAAAGGGGTTTGAGATGATTTGGGATCCTA4420.10306201441935513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG1050.095.227211
CGTAGCG254.5504748E-795.22721
CGTAGTA201.7431195E-594.994066
TAGGGTA1050.094.994063
TAGTGCA1100.094.994068
GTAGTCC409.094947E-1294.994067
GTATTAC301.2307282E-894.994057
AGTAGTC353.2923708E-1094.994056
CGTAGAG426500.093.887551
AGTACAG422700.093.870399
TAGTACA418350.093.7905968
GAGTAGT422400.093.577245
GTAGTAC420750.093.537827
AGTAGTA421950.093.474426
GTAGAGT424850.093.328282
AGAGTAG424100.093.2917254
TAGAGTA427500.092.59423
GTGGTAC1200.091.0359657
TGGTACA1200.091.0359658
AGTAGTG1200.087.077886
Produced by FastQC (version 0.11.5)