FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC333CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC333CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63553
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACGACTACGCGAGACACAGGCAAATGGTAACAACAACCAATCCAT1860.29266911082088964No Hit
CGACGACGACTACGCGAGACACAATCCCAATTATACTTGCGGCAACAACA1830.2879486412915205No Hit
CGACGACGACTACGCGAGACACAGTAGAAACAAGGTAGTTTTTTACTCTA1580.2486113952134439No Hit
CGACGACGACTACGCGAGACACAGTAGAAACAAGGTACTTTTTTGGACAG1490.2344499866253363No Hit
CGACGACGACTACGCGAGACACATTCCATGGGGCCAAAGAAATAGCACTC1350.21242112882161346No Hit
CGACGACGACTACGCGAGACACCCAAGATCAAAGGTCCCAGGTTCCAGAT1250.1966862303903828No Hit
CGACGACGACTACGCGAGACACCCAATTTTCATTATGAGGAATGTTCCTG1140.17937784211602914No Hit
CGACGACGACTACGCGAGACACAGGCCAATGGTAACAACAACCAATCCAT1100.1730838827435369No Hit
CGACGACGACTACGCGAGACACAAGAGCCAGTTGGTGTGGATGGCATGCA1080.16993690305729078No Hit
CGACGACGACTACGCGAGACACATTAGCTTTTTCCCCTTTCGCAAGGTTG1050.1652164335279216No Hit
CGACGACGACTACGCGAGACACAGGCACATGGTAACAACAACCAATCCAT1050.1652164335279216No Hit
CGACGACGACTACGCGAGACACTGAAACGAAAACGTTCTAATCTCTTGTT1010.15892247415542932No Hit
CGACGACGACTACGCGAGACACCCAAGGTCGATTTGAACCATGCCAGTTG1010.15892247415542932No Hit
CGACGACGACTACGCGAGACACAAGGGTGTTTTTTATCACTAAATAAGCT870.13689361635170647No Hit
CGACGACGACTACGCGAGACACGAAAACGTTCTAATCTCTTGTTCCACTT870.13689361635170647No Hit
CGACGACGACTACGCGAGACACAAAGAGGGCCAGCCACTTTTTGCTTGGG850.13374663666546033No Hit
CGACGACGACTACGCGAGACACCAGAGAACTGGCGACTGTTGAGTAGATC830.13059965697921422No Hit
CGACGACGACTACGCGAGACACAATAGCATCCATGTTTTCATTTGAAGCA830.13059965697921422No Hit
CGACGACGACTACGCGAGACACTGGCATAGTGATGTGTGTATGCAGGGAC710.11171777886173745No Hit
CGACGACGACTACGCGAGACACAATATCAAGTGCACAATCCCAATTATAC670.10542381948924519No Hit
CGACGACGACTACGCGAGACACAGGGGGGCAAAGACATAATGGATTCCCA660.10385032964612213No Hit
CGACGACGACTACGCGAGACACCCGACGACGACTACGCGTGATCAGTAGA640.100703349959876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTACG68450.086.809388
GACGACT68450.086.67065
ACGACTA68350.086.658426
ACTACGC68550.086.613469
CGACTAC68500.086.607337
GACGACG75100.079.312282
ACGACGA75850.078.40283
GCGACTA200.00206524871.248226
GGACTAC200.00206524871.248227
GCTACGC200.00206524871.248229
GGCTACG200.00206524871.248228
CGAAGAC250.004953419357.133441
AGGACTA250.005000023656.9985856
CGAATAC250.005000023656.9985857
GACGAAT250.005000023656.9985855
GAATACG250.005000023656.9985858
ACGGCTA250.005000023656.9985856
ACGAATA250.005000023656.9985856
CTCTGCG252.8131259E-547.4988270-71
GCGGGCC252.8131259E-547.4988274-75
Produced by FastQC (version 0.11.5)