FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC331CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC331CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33469
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGTAGTTT5151.5387373390301473No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGCCTATCA4111.2280020317308553No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTCTATCA3811.1383668469329828No Hit
CGTACTCTCGCATAGACTACTAAGGCCCTCCTTTCAGTCCGTATTTAAAG3441.0278167856822733No Hit
CGTACTCTCGCATAGACTACTACGGCACTCCTTCCGTAGAAGGCCCTCCT3060.9142788849383011No Hit
CGTACTCTCGCATAGACTACTACAGCTCTATGCTGACAAAATGACCATCG1820.5437867877737608No Hit
CGTACTCTCGCATAGACTACTAAGGCACTCCTTCCGTAGAAGGCCCTCCT1730.516896232334399No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGGTGTTT1730.516896232334399No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTAGATAT1540.460127281962413No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGCAGTTT1360.40634617108368937No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGGTGACAA1040.3107353072992919No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGCAGATAT1010.3017717888195046No Hit
CGTACTCTCGCATAGACTACTACGGCTCTATGCTGACAAAATGACCATCG990.29579610983297977No Hit
CGTACTCTCGCATAGACTACTACGGCCCTCCTTTCAGTCCGTATTTAAAG770.2300636409812065No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGGAAGTGAGGAATGATGATGTT680.20317308554184468No Hit
CGTACTCTCGCATAGACTACTAGCCGCAAATATCATTGGGATCTTGCACT670.2001852460485823No Hit
CGTACTCTCGCATAGACTACTACGGCGCTCCTTCCGTAGAAGGCCCTCCT640.191221727568795No Hit
CGTACTCTCGCATAGACTACTAATGGGGGTGCAGATGCAACGGTTCAAGT530.15835549314290837No Hit
CGTACTCTCGCATAGACTACTAGGGGCGGGAACAATTAGGTCAGAAGTTT500.1493919746631211No Hit
CGTACTCTCGCATAGACTACTAGGGCACTCCTTCCGTAGAAGGCCCTCCT460.1374406166900714No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGCGTTTT460.1374406166900714No Hit
CGTACTCTCGCATAGACTACTAGGGGGCCAAATCAGCATACAACCTACGT450.13445277719680898No Hit
CGTACTCTCGCATAGACTACTACCGCAAATATCATTGGGATCTTGCACTT450.13445277719680898No Hit
CGTACTCTCGCATAGACTACTAGGGCCGCAAATATCATTGGGATCTTGCA440.13146493770354656No Hit
CGTACTCTCGCATAGACTACTACGCCGCAAATATCATTGGGATCTTGCAC440.13146493770354656No Hit
CGTACTCTCGCATAGACTACTAAGGCCCCCTCAAAGCCGAGATCGCACAG430.12847709821028416No Hit
CGTACTCTCGCATAGACTACTAGGGGCCGCAAATATCATTGGGATCTTGC420.1254892587170217No Hit
CGTACTCTCGCATAGACTACTAGGGGGAAGAATATCGAAAGGAACAGCAG410.1225014192237593No Hit
CGTACTCTCGCATAGACTACTACGGCCGCAAATATCATTGGGATCTTGCA410.1225014192237593No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGGGTTTAA400.11951357973049688No Hit
CGTACTCTCGCATAGACTACTAAGCCGCAAATATCATTGGGATCTTGCAC390.11652574023723444No Hit
CGTACTCTCGCATAGACTACTACGGGCGGGAACAATTAGGTCAGAAGTTT380.11353790074397205No Hit
CGTACTCTCGCATAGACTACTATGCGTGATCAGCAAAAGCAGGTCTATCA380.11353790074397205No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTGTTTAA360.10756222175744719No Hit
CGTACTCTCGCATAGACTACTAGAAGGCCCTCCTTTCAGTCCGTATTTAA360.10756222175744719No Hit
CGTACTCTCGCATAGACTACTAATTAGCCTAATATTGCAAATAGGGAATA350.10457438226418476No Hit
CGTACTCTCGCATAGACTACTAACGCGTGATCAGTAGAAACAAGGTAGTT340.10158654277092234No Hit
CGTACTCTCGCATAGACTACTAAGGCGCTCCTTCCGTAGAAGGCCCTCCT340.10158654277092234No Hit
CGTACTCTCGCATAGACTACTAGGAGGGCCTTCTACGGAAGGAGTGCCAA340.10158654277092234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA33450.093.435038
CGCATAG33450.093.435039
CTCGCAT33350.093.430347
ACTCTCG33650.093.3031544
TCTCGCA33550.093.014956
CGTACTC35650.088.332831
GTACTCT35750.087.955282
TACTCTC35750.087.955283
CTCTCGC37050.084.484525
CTACGGC2250.047.4985120-21
ACTACGG4300.047.4985118-19
CTACACG452.2009772E-1047.49850520-21
TAGCGGG301.4714642E-647.49850520-21
ACTAATG404.123649E-947.49850518-19
ACTAAGC600.047.49850518-19
ACTAACG950.047.49850518-19
ACTAACC501.0913936E-1147.49850518-19
ACTAAAG404.123649E-947.49850518-19
ACTAAAC205.3524825E-447.49850518-19
ACTAAAA404.123649E-947.49850518-19
Produced by FastQC (version 0.11.5)