FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC330CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC330CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63124
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATATAGTACTGTCGCTGGGGGGCTATATGAAGCAATTGAGGAGTG3670.5813953488372093No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGAAAGATCCTAAGAAAACTGGA2780.4404030162854065No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAAGATCCTAAGAAAACTGG2270.35960965718268806No Hit
CGAGTATATAGTACTGTCGCTGAAGATAACAGAGAATAGTTTTGAGCAAA2090.3310943539699639No Hit
CGAGTATATAGTACTGTCGCTGGCGGGAACAATTAGGTCAGAAGTTTGAA2080.32951017045814585No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAATGGACGAAAAACAAGAAT1960.3104999683163298No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGCCTGACGGGATGATAGAGAGTA1850.29307394968633166No Hit
CGAGTATATAGTACTGTCGCTGAGGGGGCCTGACGGGATGATAGAGAGTA1770.2804004815917876No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAACAATTAGGTCAGAAGTTT1660.2629744629617895No Hit
CGAGTATATAGTACTGTCGCTGGGGGCGGGAACAATTAGGTCAGAAGTTT1490.23604334326088333No Hit
CGAGTATATAGTACTGTCGCTGGGGGGAACAATTAGGTCAGAAGTTTGAA1320.2091122235599772No Hit
CGAGTATATAGTACTGTCGCTGGGGGTGCAGATGCAACGGTTCAAGTGAT1310.2075280400481592No Hit
CGAGTATATAGTACTGTCGCTGAGAAGCAGTAATGAGAATGGGAGACCTC1130.179012736835435No Hit
CGAGTATATAGTACTGTCGCTGGGGGGCCTGACGGGATGATAGAGAGTAC1130.179012736835435No Hit
CGAGTATATAGTACTGTCGCTGGGGGGAAGAATATCGAAAGGAACAGCAG1120.177428553323617No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAATATCGAAAGGAACAGCAG1110.175844369811799No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGAGAATATCGAAAGGAACAGCAG1070.16950763576452696No Hit
CGAGTATATAGTACTGTCGCTGGGGGAGGGAAGAATATCGAAAGGAACAG1050.16633926874089094No Hit
CGAGTATATAGTACTGTCGCTGGAGGGAGGAATATCGAAAGGAACAGCAG1040.16475508522907292No Hit
CGAGTATATAGTACTGTCGCTGGGGGGAGGAATATCGAAAGGAACAGCAG990.1568341676699829No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGTGAGAATGGACGAAAAACAAGA950.15049743362271084No Hit
CGAGTATATAGTACTGTCGCTGGAGGGCCTTCTACGGAAGGAGTGCCAAA940.14891325011089285No Hit
CGAGTATATAGTACTGTCGCTGCAAAGACATCTTCAAGTCTCTGTGCGAT890.14099233255180282No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAATCTTCGAGCTCTCGGACG880.13940814903998477No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGTCTTCGAGCTCTCGGAC870.13782396552816678No Hit
CGAGTATATAGTACTGTCGCTGCAACTCGTTTGCGGACATGCCAAAGAAA850.13465559850453077No Hit
CGAGTATATAGTACTGTCGCTGGGTTGAATTAATCAGGGGACGACCTAAA840.13307141499271274No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGAAAATAAAAACAACCAAAATGA810.12831886445725874No Hit
CGAGTATATAGTACTGTCGCTGAGGGAAGAATATCGAAAGGAACAGCAGA790.1251504974336227No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGTGAGGAATGATGATGTT780.1235663139218047No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAACTATTACTGGACCTTGCT780.1235663139218047No Hit
CGAGTATATAGTACTGTCGCTGGGGGCCTGACGGGATGATAGAGAGTACG760.12039794689816868No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGAGAATGGACGAAAAACAAGAAT760.12039794689816868No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGCTATATGAAGCAATTGAGGAGT750.11881376338635068No Hit
CGAGTATATAGTACTGTCGCTGCTGGGTTGAATTAATCAGGGGACGACCT740.11722957987453267No Hit
CGAGTATATAGTACTGTCGCTGAGGAGCTGAGGGAGCAATTGAGCTCAGT730.11564539636271466No Hit
CGAGTATATAGTACTGTCGCTGGGGGGCCAAAGTCTATGAGGGAAGAATA720.11406121285089665No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGCCTGACGGGATGATAGAGAGT720.11406121285089665No Hit
CGAGTATATAGTACTGTCGCTGGAGGGGAGAATATCGAAAGGAACAGCAG710.11247702933907865No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGAATATCGAAAGGAACAG660.1045561117799886No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGCCTGACGGGATGATAGAGAG650.10297192826817059No Hit
CGAGTATATAGTACTGTCGCTGGAGGAGGGAAGAATATCGAAAGGAACAG640.10138774475635258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTA156.5895973E-494.996834
AAGTACT156.5895973E-494.996839
TATATTA156.5895973E-494.996837
TATATGG156.5895973E-494.996835
TGGTACT156.5895973E-494.996839
GTGTATA156.5895973E-494.996834
GATATAG301.2132659E-894.996835
AGGATAT301.2132659E-894.996833
TATAAAG156.5895973E-494.996835
ATGGTAC156.5895973E-494.996838
GTATAGT156.5895973E-494.996836
AGTATGT156.5895973E-494.996833
GTATAAA156.5895973E-494.996834
TAGTAAT156.5895973E-494.996839
ATAAAGT156.5895973E-494.996836
TGTAGTA156.5895973E-494.996837
ATGTAGT156.5895973E-494.996836
AAAGTAC156.5895973E-494.996838
TAAAGTA156.5895973E-494.996837
ATATTAC156.5895973E-494.996838
Produced by FastQC (version 0.11.5)