FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC324CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC324CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91721
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAGTCTCTATACGTCTAGCGGGGGTTTTGTTCAGCATCCAGAACTAA2870.3129054415019461No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGGAAAATGGACGAAGGACAAGG2710.29546123570392824No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGGAACAGTGACCACAGAAGCTG2420.26384361269502077No Hit
CGTCAGTCTCTATACGTCTAGCCGGCGCCATTCTGTTTTCATGCCTGATT2350.25621177265838796No Hit
CGTCAGTCTCTATACGTCTAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA2030.22132336106235212No Hit
CGTCAGTCTCTATACGTCTAGCCGGCACCATTCTGTTTTCATGCCTGATT2030.22132336106235212No Hit
CGTCAGTCTCTATACGTCTAGCAGGCCCCCTCAAAGCCGAGATCGCGCAG2010.2191428353375999No Hit
CGTCAGTCTCTATACGTCTAGCGAGGCCAAGGGTGTTGCCTCTTCCTTTT1770.19297652664057302No Hit
CGTCAGTCTCTATACGTCTAGCAGAAGCGAATGGGAGTGCAGATGCAGCG1750.1907960009158208No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGAAAATGGACGAAGGACAAGGG1460.15917837790691336No Hit
CGTCAGTCTCTATACGTCTAGCGGGAGTTTTGTTCAGCATCCAGAACTAA1430.155907589319785No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGAAAATGGACGAAGGACAAGGGT1420.1548173264574089No Hit
CGTCAGTCTCTATACGTCTAGCCCGACCAGTGAGTACCCTTCCCTTTCAA1350.14718548642077606No Hit
CGTCAGTCTCTATACGTCTAGCAGGGGGGGAACAGTGACCACAGAAGCTG1330.1450049606960238No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGCCAAGGAGGTGTCACTAAGCTA1290.14064390924651932No Hit
CGTCAGTCTCTATACGTCTAGCAAAAGCAGGTAGATATTTAAAGATGAGT1240.13519259493463875No Hit
CGTCAGTCTCTATACGTCTAGCCAGAGCAGAAATGAAAAGTGGCGAGAGC1230.13410233207226263No Hit
CGTCAGTCTCTATACGTCTAGCCGCCCAAGATCGAAGGTTCCAGGTTCCA1180.12865101776038204No Hit
CGTCAGTCTCTATACGTCTAGCCGCCTCAAAGCCGAGATCGCGCAGAGAC1170.12756075489800592No Hit
CGTCAGTCTCTATACGTCTAGCATGAGCATGAACCAGTCTCGTGACATTT1130.12319970344850144No Hit
CGTCAGTCTCTATACGTCTAGCCGGACCAGTGAGTACCCTTCCCTTTCAA1100.11992891486137308No Hit
CGTCAGTCTCTATACGTCTAGCCGGAGCAGAAATGAAAAGTGGCGAGAGC1050.11447760054949248No Hit
CGTCAGTCTCTATACGTCTAGCGAGGGGGGAAAATGGACGAAGGACAAGG1040.11338733768711637No Hit
CGTCAGTCTCTATACGTCTAGCAGCAAATATCATTGGGATCTTGCACCTG1000.10902628623761189No Hit
CGTCAGTCTCTATACGTCTAGCGGGGCCAAGGGTGTTGCCTCTTCCTTTT1000.10902628623761189No Hit
CGTCAGTCTCTATACGTCTAGCGGGGACAGAAATTTGAGGAAATAAGGTG980.10684576051285964No Hit
CGTCAGTCTCTATACGTCTAGCCAGCACCATTCTGTTTTCATGCCTGATT970.10575549765048353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT254.5425077E-795.1540151
CATCAGT254.5425077E-795.1540151
GAGTCTC254.587073E-794.998374
GTTAGTC254.587073E-794.998372
TCGATAC156.5988046E-494.998379
TGTCTAT156.5988046E-494.998377
GTGAGTC254.587073E-794.998372
TTAGTCT254.587073E-794.998373
TCTATGC156.5988046E-494.998379
TCAGGCT201.7353956E-594.998363
TCTATAG201.7353956E-594.998369
CGTCAGT90200.094.5738141
TCAGTCT90550.094.421353
GTCAGTC90400.094.420392
CTCTATA90350.094.4200748
TCTATAC90200.094.419119
TCTCTAT90850.094.004987
GTCTCTA91250.093.905236
AGTCTCT92200.092.937675
CAGTCTC93150.091.632884
Produced by FastQC (version 0.11.5)