FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC320CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC320CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31880
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTAGTCGCGTGTACGTCGCTACGCGTGATCAGCAAAAGCAGGTGGCAC2070.6493099121706399No Hit
CGCTAGTCGCGTGTACGTCGCTACGCGTGATCAGCAAAAGCAGGCGGCAC1930.6053952321204517No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGGAAATGAAGCAGGGGGCTGCGC1580.4956085319949812No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGGGAAATGAAGCAGGGGGCTGCG1270.39836888331242154No Hit
CGCTAGTCGCGTGTACGTCGCTGCGCGTGATCAGCAAAAGCAGGTGGCAC900.2823086574654956No Hit
CGCTAGTCGCGTGTACGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGT890.2791718946047679No Hit
CGCTAGTCGCGTGTACGTCGCTCCGCGTGATCAGCAAAAGCAGGTGGCAC880.27603513174404015No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGTTGTAGTTGGCGGCAAGATTATTA850.266624843161857No Hit
CGCTAGTCGCGTGTACGTCGCTGGCCCAGCAGCCATGCCTTCTAAGCAGT730.22898368883312423No Hit
CGCTAGTCGCGTGTACGTCGCTCTTCGAAATGGATGGCACTTTGATTGAA600.18820577164366373No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGAAGAATATCAACAGGAACAGCAG550.1725219573400251No Hit
CGCTAGTCGCGTGTACGTCGCTCCGCGTGATCAGCAAAAGCAGGCGGCAC540.16938519447929737No Hit
CGCTAGTCGCGTGTACGTCGCTGAGGGAAGAATATCAACAGGAACAGCAG530.16624843161856964No Hit
CGCTAGTCGCGTGTACGTCGCTGCGCGTGATCAGCAAAAGCAGGCGGCAC480.150564617314931No Hit
CGCTAGTCGCGTGTACGTCGCTAGGCGTGATCAGCAAAAGCAGGTGGCAC460.14429109159347553No Hit
CGCTAGTCGCGTGTACGTCGCTGTCAGTAAGTATGCTAGAGTCCCGTTTT430.13488080301129235No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGGCAAGAAGGGGATTGAACTCAG430.13488080301129235No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGTGCAGCCCACCCCTCTCATTTG400.12547051442910914No Hit
CGCTAGTCGCGTGTACGTCGCTAAGGGGGCAAGGGGACGTAGTGTTGGTA400.12547051442910914No Hit
CGCTAGTCGCGTGTACGTCGCTATGCGTGATCAGCAAAAGCAGGTGGCAC390.12233375156838143No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGGGAAGCTTCGTGCTGGAGAAAA370.11606022584692598No Hit
CGCTAGTCGCGTGTACGTCGCTCTCAGAATGAAGTGGATGATGGCAATGA350.10978670012547051No Hit
CGCTAGTCGCGTGTACGTCGCTGGCGGGGAAATGAAGCAGGGGGCTGCGC350.10978670012547051No Hit
CGCTAGTCGCGTGTACGTCGCTCGGCGTGATCAGCAAAAGCAGGTGGCAC350.10978670012547051No Hit
CGCTAGTCGCGTGTACGTCGCTAAGACCACTGTGGACCATATGGCCATAA340.1066499372647428No Hit
CGCTAGTCGCGTGTACGTCGCTGGGTGCTGGGGTTGTAGTTGGCGGCAAG340.1066499372647428No Hit
CGCTAGTCGCGTGTACGTCGCTCGGGGGGGAAATGAAGCAGGGGGCTGCG340.1066499372647428No Hit
CGCTAGTCGCGTGTACGTCGCTACGCGTGATCAGTAGAAACAAGGTCGTT330.10351317440401506No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGAGAATATCAACAGGAACAGCAG330.10351317440401506No Hit
CGCTAGTCGCGTGTACGTCGCTCGGGGGGAAATGAAGCAGGGGGCTGCGC320.10037641154328732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG156.55892E-494.9984364
AGTCGGG156.55892E-494.9984365
CTAGTCG32050.093.5163963
GTCGCGT31900.093.360536
CGCGTGT31750.093.352798
TAGTCGC32000.093.217214
GCGTGTA31750.093.2031869
AGTCGCG32050.093.0717855
TCGCGTG32100.092.926817
CGCTAGT32050.092.921251
GCTAGTC32250.092.789162
GGGTGTA250.004977009356.9990589
GTCGGGT250.004977009356.9990586
TCGGGTG250.004977009356.9990587
CGCTGGA1250.047.49921818-19
TGCGCGT252.7970664E-547.49921822-23
GGCGTGA600.047.49921824-25
CGCTCTG501.0913936E-1147.49921818-19
CGCTCTC301.4701927E-647.49921818-19
CGCTCGG2450.047.49921818-19
Produced by FastQC (version 0.11.5)