FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC317CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC317CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55690
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGATATAGTCAGAGAGTAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA1760.316035194828515No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGGAAAATGGACGAAGGACAAGG1330.23882205063745737No Hit
CGTCGATATAGTCAGAGAGTAGGGGGCCTGACGGGATGATAGAAAGAACG1240.22266115999281738No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGAGCGCCAGGATGCCACAGAAATC1170.21009157838031964No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCAAAAACATAATGGACTCCAACA1090.19572634225175078No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCTATATGAAGCAATCGAGGAGTG900.16160890644639972No Hit
CGTCGATATAGTCAGAGAGTAGCGGCGCGAAGTAGGTACAGATGCAATTG810.14544801580175976No Hit
CGTCGATATAGTCAGAGAGTAGAGGCCCATTAGAGCACATCCAGAAACTG790.1418567067696175No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCAGAAATTTGAGGAAATAAGGTG760.1364697432214042No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGCAAAAACATAATGGACTCCAA760.1364697432214042No Hit
CGTCGATATAGTCAGAGAGTAGCGGCGAAGTAGGTACAGATGCAATTGTC760.1364697432214042No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCGAAAACATAATGGACTCCAACA730.13108277967319087No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGAGCGCCAGGATGCCACAGAAAT710.12749147064104865No Hit
CGTCGATATAGTCAGAGAGTAGAGGCGCGAAGTAGGTACAGATGCAATTG700.12569581612497754No Hit
CGTCGATATAGTCAGAGAGTAGCCGCGAAGTAGGTACAGATGCAATTGTC660.11851319806069312No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGCAAAAACATAATGGACTCCAAC650.11671754354462202No Hit
CGTCGATATAGTCAGAGAGTAGGAGGGGGGAAAATGGACGAAGGACAAGG620.11133057999640869No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGCAATAGTTGGAGAAATTTCAC620.11133057999640869No Hit
CGTCGATATAGTCAGAGAGTAGAGGAGCTAAGAGAGCAATTGAGCTCAGT610.1095349254803376No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCGGAAATTTGAGGAAATAAGGTG590.10594361644819536No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGAAAGCTGGAATCAACAAGGAT570.10235230741605315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT201.7069637E-595.247741
CATCGAT254.492358E-795.247741
TGATATA201.7300345E-594.991014
ATCGATA254.5652996E-794.991012
CGTCGAT54800.094.639411
ATATAGT55050.094.387076
CGATATA54950.094.385984
GTCGATA54950.094.385982
TATAGTC55000.094.300177
TAGTCAG55050.094.2145169
GATATAG55300.094.046265
ATAGTCA55350.093.446448
TCGATAT55700.093.285613
GTTGATA255.2337877E-575.9928052
TTGATAT255.2337877E-575.9928053
GTATTTC205.345844E-447.5382262-63
TTGGTAT205.345844E-447.5382260-61
GTAGAGG4550.047.4955118-19
TAGAGGG1950.047.4955120-21
TAGAGGC1900.047.4955120-21
Produced by FastQC (version 0.11.5)