FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC316CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC316CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80849
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACTCACGCGCGACACGAGGCCAAGGGTGTTGCCTCTTCCTTTTA4710.5825675023809819No Hit
CGACATACTCACGCGCGACACGATCAGCAAAAGCAGGGTGACAAAAACAT2420.2993234300980841No Hit
CGACATACTCACGCGCGACACGCGGCCAAGGGTGTTGCCTCTTCCTTTTA1990.24613786194016005No Hit
CGACATACTCACGCGCGACACGAACAGAGGTTATAAGAATGATGGAAAGT1840.2275847567687912No Hit
CGACATACTCACGCGCGACACGGGGCCAAGGGTGTTGCCTCTTCCTTTTA1640.2028472832069661No Hit
CGACATACTCACGCGCGACACGATCAGCAAAAGCAGGAGTTTAAAATGAA1150.1422404729804945No Hit
CGACATACTCACGCGCGACACGATGCAATTGTCATTCTAAGTGTCTCGCT1000.12368736780912566No Hit
CGACATACTCACGCGCGACACGGAAACCACTGACTGTCCAGCAGCAAAGT960.11873987309676062No Hit
CGACATACTCACGCGCGACACGAAAAGCAGGGTGACAAAAACATAATGGA920.1137923783843956No Hit
CGACATACTCACGCGCGACACGAGGGGGCCTGACGGGATGATAGAAAGAA920.1137923783843956No Hit
CGACATACTCACGCGCGACACGCGTGATCAGTAGAAACAAGGGTGTTTTT880.10884488367203057No Hit
CGACATACTCACGCGCGACACGACAACATGGATAGAGCAGTTAAACTATA830.1026605152815743No Hit
CGACATACTCACGCGCGACACGGTGGGGAGCGCCAGGATGCCACAGAAAT830.1026605152815743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG156.5961684E-494.997537
TAACGCG156.5961684E-494.997539
TACGCAC201.7342152E-594.997536
ACATACG201.7342152E-594.997533
CTAACGC156.5961684E-494.997538
CTCACTC156.5961684E-494.997538
CATACGC201.7342152E-594.997534
ACAGACT254.5823435E-794.997523
GACTCAC254.5823435E-794.997526
ACTCACG80750.093.644627
CTCACGC80650.093.6429448
TCACGCG80800.093.46919
TACTCAC80950.093.413256
ATACTCA81350.092.8955465
CGACATA81450.092.7778851
CATACTC81300.092.718994
GACATAC81900.092.44572
ACATACT81750.092.4410253
TGACATA200.00204705671.424941
TCACGGG200.00206729771.2481469
Produced by FastQC (version 0.11.5)