FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC313CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC313CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87035
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGTCGTACTCAGTGATAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA5830.6698454644683174No Hit
CGATAGTCGTACTCAGTGATAGGGGGCCTGACGGGATGATAGAAAGAACG5130.5894180502096857No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAAATGGACGAAGGACAAGG4990.5733325673579595No Hit
CGATAGTCGTACTCAGTGATAGCGGCACAGATTGAAAGCGACAGAGAATA3530.4055839604756707No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCTATATGAAGCAATCGAGGAGTG3340.38375366231975644No Hit
CGATAGTCGTACTCAGTGATAGCGGCCAAGGGTGTTGCCTCTTCCTTTTA2790.32056069397368875No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAGGCTCTATTGGGAAAGTG2690.3090710633653128No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAATAGTTGGAGAAATTTCAC2680.3079221003044752No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAGGAGTTGAACCAAGATGC2580.29643246969609927No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGAAAATGGACGAAGGACAAGGG1980.2274946860458436No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCAGATTGAAAGCGACAGAGAATA1750.20106853564657895No Hit
CGATAGTCGTACTCAGTGATAGAGGCCCATTAGAGCACATCCAGAAACTG1320.15166312403056242No Hit
CGATAGTCGTACTCAGTGATAGAGGCCCATGCAACTGGCAAGTGCACCAG1120.12868386281381053No Hit
CGATAGTCGTACTCAGTGATAGGGTGGTTAATTGAAGAAATGCGGCACAG1100.12638593669213535No Hit
CGATAGTCGTACTCAGTGATAGGGGGGAAGAATATCAACAGGAACAGCAG1090.12523697363129777No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGCAAAAACATAATGGACTCCAAC1080.12408801057046015No Hit
CGATAGTCGTACTCAGTGATAGGAGGGGGGAAAATGGACGAAGGACAAGG1050.12064112138794737No Hit
CGATAGTCGTACTCAGTGATAGAGGGGGCCTGACGGGATGATAGAAAGAA1010.11604526914459701No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAAAAACATAATGGACTCCAACA1000.1148963060837594No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCTGGTGTTTATAGCACCCTTGGG940.10800252771873385No Hit
CGATAGTCGTACTCAGTGATAGGGGGCGGGAAGTCTTCGAGCTCTCGGAC930.10685356465789625No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCCTGACGGGATGATAGAAAGAAC920.10570460159705865No Hit
CGATAGTCGTACTCAGTGATAGGGGGCCAAGGAGGTGTCACTAAGCTATT910.10455563853622107No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAGAAATTTGAGGAAATAAGGTG910.10455563853622107No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAAAATTAAACAGAGAAGAAATA900.10340667547538347No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAAAAACATAATGGACTCCAA900.10340667547538347No Hit
CGATAGTCGTACTCAGTGATAGCGAGGCCAAGGGTGTTGCCTCTTCCTTT880.10110874935370828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGT201.7353694E-594.99314
TTGTACT254.5865818E-794.99317
GAGAGTC201.7353694E-594.99312
TCGGACT201.7353694E-594.99317
GTCGTAT201.7353694E-594.99316
GTCGTAG201.7353694E-594.99316
TCGTATT201.7353694E-594.99317
TCGTAGT201.7353694E-594.99317
TGTACTC254.5865818E-794.99318
AGTACTC201.7353694E-594.99318
GTAGTCA201.7353694E-594.99319
GTAGTAC201.7353694E-594.99316
GTCGGAC201.7353694E-594.99316
GTACCCA156.599109E-494.9930959
GTCGAAC301.2190867E-894.9930956
GTCGTAA156.599109E-494.9930956
GTACTAA156.599109E-494.9930959
CGTACCC156.599109E-494.9930958
TCGTACC156.599109E-494.9930957
TCGTAAT156.599109E-494.9930957
Produced by FastQC (version 0.11.5)