FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC310CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC310CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15535
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTATACGACGTAGTATATCTCTGCGCGATCTCGGCTTTGAGGGGGCCT3262.0984872867718054No Hit
CGCTATACGACGTAGTATATCTGGGGGAAGAATATCAACAGGAACAGCAG1701.0943031863533954No Hit
CGCTATACGACGTAGTATATCTAGGCCAAGGGTGTTGCCTCTTCCTTTTA1370.8818796266495013No Hit
CGCTATACGACGTAGTATATCTGAGGGAAGAATATCAACAGGAACAGCAG1220.7853234631477308No Hit
CGCTATACGACGTAGTATATCTGGGGGGGGAAAATGGACGAAGGACAAGG1070.6887672996459607No Hit
CGCTATACGACGTAGTATATCTAACCGAGGTCGAAACGTACGTTCTTTCT1040.6694560669456067No Hit
CGCTATACGACGTAGTATATCTGGGGGCAATCAGTTTCTGGATGTGCTCT850.5471515931766977No Hit
CGCTATACGACGTAGTATATCTCGGCGCGATCTCGGCTTTGAGGGGGCCT600.3862246540070808No Hit
CGCTATACGACGTAGTATATCTGAGGGGAGAATATCAACAGGAACAGCAG590.3797875764402961No Hit
CGCTATACGACGTAGTATATCTACGCGTGATCAGTAGAAACAAGGGTGTT560.36047634373994203No Hit
CGCTATACGACGTAGTATATCTGAGGGAGGAATATCAACAGGAACAGCAG530.341165111039588No Hit
CGCTATACGACGTAGTATATCTGAGAGGCCAAGGGTGTTGCCTCTTCCTT500.32185387833923396No Hit
CGCTATACGACGTAGTATATCTCGGGACAGAAATTTGAGGAAATAAGGTG470.30254264563887995No Hit
CGCTATACGACGTAGTATATCTGGAGGGGGAAAATGGACGAAGGACAAGG460.2961055680720953No Hit
CGCTATACGACGTAGTATATCTCGAACAAATAACATTTATGCAAGCCTTA450.28966849050531057No Hit
CGCTATACGACGTAGTATATCTGGGGACAGAAATTTGAGGAAATAAGGTG430.27679433537174125No Hit
CGCTATACGACGTAGTATATCTAGGGGAAGAATATCAACAGGAACAGCAG370.23817186997103315No Hit
CGCTATACGACGTAGTATATCTAAGGGAAGAATATCAACAGGAACAGCAG360.2317347924042485No Hit
CGCTATACGACGTAGTATATCTGCGCGATCTCGGCTTTGAGGGGGCCTGA360.2317347924042485No Hit
CGCTATACGACGTAGTATATCTGGGAGCCGAACAACATGGATAGAGCAGT360.2317347924042485No Hit
CGCTATACGACGTAGTATATCTGGGACGGAAATTTGAGGAAATAAGGTGG350.22529771483746378No Hit
CGCTATACGACGTAGTATATCTCGAGGCCAAGGGTGTTGCCTCTTCCTTT350.22529771483746378No Hit
CGCTATACGACGTAGTATATCTCGGCTTTGAGGGGGCCTGACGGGATGAT340.21886063727067911No Hit
CGCTATACGACGTAGTATATCTGGGAGGCCAAGGGTGTTGCCTCTTCCTT340.21886063727067911No Hit
CGCTATACGACGTAGTATATCTCCAACACCATGTCAAGCTTTCAGGTAGA330.21242355970389443No Hit
CGCTATACGACGTAGTATATCTCAGGAGGGAAGAATATCAACAGGAACAG320.20598648213710977No Hit
CGCTATACGACGTAGTATATCTCAGGGAAGAATATCAACAGGAACAGCAG320.20598648213710977No Hit
CGCTATACGACGTAGTATATCTCGGGGAAGAATATCAACAGGAACAGCAG310.19954940457032505No Hit
CGCTATACGACGTAGTATATCTACGCGTGATCAGCAAAAGCAGGTAGATA300.1931123270035404No Hit
CGCTATACGACGTAGTATATCTGAAACGAGAAAGCTCTTATCTCTTGTTC290.1866752494367557No Hit
CGCTATACGACGTAGTATATCTAACCGAGGTCGAAACGTACGTTCTTTCC290.1866752494367557No Hit
CGCTATACGACGTAGTATATCTCAGAGGCCAAGGGTGTTGCCTCTTCCTT290.1866752494367557No Hit
CGCTATACGACGTAGTATATCTGAAACGGGAAAGCTCTTATCTCTTGTTC270.17380109430318635No Hit
CGCTATACGACGTAGTATATCTGAGGCCAAGGGTGTTGCCTCTTCCTTTT270.17380109430318635No Hit
CGCTATACGACGTAGTATATCTAGGGACAGAAATTTGAGGAAATAAGGTG270.17380109430318635No Hit
CGCTATACGACGTAGTATATCTGGGGGCCGAACAACATGGATAGAGCAGT270.17380109430318635No Hit
CGCTATACGACGTAGTATATCTCGGCTCTGAGGGGGCCTGACGGGATGAT270.17380109430318635No Hit
CGCTATACGACGTAGTATATCTGAGGGGGGAAAATGGACGAAGGACAAGG260.16736401673640167No Hit
CGCTATACGACGTAGTATATCTCCGAGGCCAAGGGTGTTGCCTCTTCCTT260.16736401673640167No Hit
CGCTATACGACGTAGTATATCTGGGGGCAGAAATTTGAGGAAATAAGGTG250.16092693916961698No Hit
CGCTATACGACGTAGTATATCTGACGGGATGATAGAAAGAACGTACGTTT240.15448986160283232No Hit
CGCTATACGACGTAGTATATCTAGGACAGAAATTTGAGGAAATAAGGTGG240.15448986160283232No Hit
CGCTATACGACGTAGTATATCTGGGGGAGGAATATCAACAGGAACAGCAG230.14805278403604766No Hit
CGCTATACGACGTAGTATATCTGGGGGACAAAAACATAATGGACTCCAAC230.14805278403604766No Hit
CGCTATACGACGTAGTATATCTGCAACAGGAACAGCAGAGTGCTGTGGAT230.14805278403604766No Hit
CGCTATACGACGTAGTATATCTCGGACAGAAATTTGAGGAAATAAGGTGG230.14805278403604766No Hit
CGCTATACGACGTAGTATATCTGGGACAGAAATTTGAGGAAATAAGGTGG230.14805278403604766No Hit
CGCTATACGACGTAGTATATCTGAAGGGGCAATAGTTGGAGAAATTTCAC220.14161570646926297No Hit
CGCTATACGACGTAGTATATCTGGGCACGGTGAGCGTGAACACAAATCCT220.14161570646926297No Hit
CGCTATACGACGTAGTATATCTAGACAGAGAATAGTTTCGAACAAATAAC220.14161570646926297No Hit
CGCTATACGACGTAGTATATCTATGCGCGATCTCGGCTTTGAGGGGGCCT220.14161570646926297No Hit
CGCTATACGACGTAGTATATCTGGGGGGCCAAGGAGGTGTCACTAAGCTA220.14161570646926297No Hit
CGCTATACGACGTAGTATATCTGGGGCAGAAATTTGAGGAAATAAGGTGG210.13517862890247828No Hit
CGCTATACGACGTAGTATATCTACGCGTGATCAGTAGAAACAAGGTAGTT210.13517862890247828No Hit
CGCTATACGACGTAGTATATCTGGGGGGGAAAATGGACGAAGGACAAGGG200.1287415513356936No Hit
CGCTATACGACGTAGTATATCTCGGCCAAGGGTGTTGCCTCTTCCTTTTA200.1287415513356936No Hit
CGCTATACGACGTAGTATATCTGGTGGGGAGCGCCAGGATGCCACAGAAA200.1287415513356936No Hit
CGCTATACGACGTAGTATATCTTGCACCTGATATTGTGGATTACTGATCG200.1287415513356936No Hit
CGCTATACGACGTAGTATATCTACAGGAACAGCAGAGTGCTGTGGATGTT200.1287415513356936No Hit
CGCTATACGACGTAGTATATCTGGGACCCGAACAACATGGATAGAGCAGT200.1287415513356936No Hit
CGCTATACGACGTAGTATATCTGGGGGGAGAATATCAACAGGAACAGCAG200.1287415513356936No Hit
CGCTATACGACGTAGTATATCTGGGACAGGAATTTGAGGAAATAAGGTGG190.12230447376890892No Hit
CGCTATACGACGTAGTATATCTGAGGGGGGAATATCAACAGGAACAGCAG180.11586739620212425No Hit
CGCTATACGACGTAGTATATCTGGCGAGGCAATTGGGACAGAAATTTGAG180.11586739620212425No Hit
CGCTATACGACGTAGTATATCTGGGGGAGCAATTGGGACAGAAATTTGAG180.11586739620212425No Hit
CGCTATACGACGTAGTATATCTGGGGGAGCAATAGTTGGAGAAATTTCAC180.11586739620212425No Hit
CGCTATACGACGTAGTATATCTGGGCCAAGGGTGTTGCCTCTTCCTTTTA180.11586739620212425No Hit
CGCTATACGACGTAGTATATCTGGGGCCCGAACAACATGGATAGAGCAGT180.11586739620212425No Hit
CGCTATACGACGTAGTATATCTCCACGCGTGATCAGTAGAAACAAGGGTG180.11586739620212425No Hit
CGCTATACGACGTAGTATATCTCAGGACAGAAATTTGAGGAAATAAGGTG170.10943031863533956No Hit
CGCTATACGACGTAGTATATCTGGGACCGGAACAACATGGATAGAGCAGT170.10943031863533956No Hit
CGCTATACGACGTAGTATATCTCGGGAGGGAAGAATATCAACAGGAACAG170.10943031863533956No Hit
CGCTATACGACGTAGTATATCTGAGGAAGGAACAATAGTTGGAGAAATTT170.10943031863533956No Hit
CGCTATACGACGTAGTATATCTGGACAGAGAATAGTTTCGAACAAATAAC170.10943031863533956No Hit
CGCTATACGACGTAGTATATCTGGCAGGGGAAAACAAAAGCAACAAAAAT160.10299324106855488No Hit
CGCTATACGACGTAGTATATCTGGGGTGGAGTCTGCTGTCCTGAGAGGAT160.10299324106855488No Hit
CGCTATACGACGTAGTATATCTGAGTCCATGAGGGAAGAATATCAACAGG160.10299324106855488No Hit
CGCTATACGACGTAGTATATCTGGCAGAGCAATTGGGACAGAAATTTGAG160.10299324106855488No Hit
CGCTATACGACGTAGTATATCTGAGGAAGGAGCAATAGTTGGAGAAATTT160.10299324106855488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA15400.094.6853561
CTATACG15350.094.37793
GCTATAC15350.094.37792
CGACGTA15250.094.373848
ACGACGT15250.094.373847
TATACGA15350.094.068474
ATACGAC15350.094.068475
TACGACG15300.094.065436
GACGTAG15550.093.164049
AACAACA404.0017767E-947.4983930-31
TCTAGGC205.307854E-447.4983920-21
CTGGGGC205.307854E-447.4983920-21
CTGGGAG205.307854E-447.4983920-21
TCTGGGA404.0017767E-947.4983920-21
TCTGGCA205.307854E-447.4983920-21
ATCTAGG800.047.4983918-19
ATCTAGC205.307854E-447.4983918-19
TCGGCGC205.307854E-447.4983922-23
CTAAGAG205.307854E-447.4983920-21
CCGCAAA205.307854E-447.4983988-89
Produced by FastQC (version 0.11.5)