FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC309CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC309CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31323
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAGTCTTCGAGCTCTCGGAC2780.887526737541104No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAATGGACGAAGGACAAGG1890.6033904798390959No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTGTTT1790.5714650576253871No Hit
CGTAGTATCTATAGTACTGCTAGGGGGAAGAATATCAACAGGAACAGCAG1400.4469559109919229No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGCAAAAACATAATGGACTCCAAC1350.43099319988506846No Hit
CGTAGTATCTATAGTACTGCTAACGCGAAGTAGGTACAGATGCAATTGTC970.30967659547297516No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGGTGACAA970.30967659547297516No Hit
CGTAGTATCTATAGTACTGCTAGGGGACAGAAATTTGAGGAAATAAGGTG850.2713660888165246No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGAAAATGGACGAAGGACAAGGGT820.26178846215241197No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAAAAACATAATGGACTCCAA790.2522108354882993No Hit
CGTAGTATCTATAGTACTGCTAGGGGCGGGAAGTCTTCGAGCTCTCGGAC740.23624812438144496No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGACAAAAACATAATGGACTCCAA740.23624812438144496No Hit
CGTAGTATCTATAGTACTGCTAGGGGGACAAAAACATAATGGACTCCAAC660.21070778661047793No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCCTGACGGGATGATAGAAAGAAC650.20751524438910704No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGTAGATAT640.20432270216773615No Hit
CGTAGTATCTATAGTACTGCTAGAAGGTTAACAGAGTGTGTTACTGTTAC610.19474507550362355No Hit
CGTAGTATCTATAGTACTGCTAGAGGGAAGAATATCAACAGGAACAGCAG600.19155253328225266No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAACAAAAGCAACAAAAAT600.19155253328225266No Hit
CGTAGTATCTATAGTACTGCTAAATCGCTTGCGGATATGCCAAAGGAAAC600.19155253328225266No Hit
CGTAGTATCTATAGTACTGCTAAGCCCATACACACAAGCAGGCAGGCAGG590.18835999106088178No Hit
CGTAGTATCTATAGTACTGCTACAGAGCAGAAATGAAAAGTGGCGAGAGC560.17878236439676914No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAATCAGTTTCTGGATGTGCT550.17558982217539826No Hit
CGTAGTATCTATAGTACTGCTAGAGGGGGGAAAATGGACGAAGGACAAGG540.1723972799540274No Hit
CGTAGTATCTATAGTACTGCTACGGGAGGGAAGAATATCAACAGGAACAG500.15962711106854388No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGTAGTTT480.15324202662580214No Hit
CGTAGTATCTATAGTACTGCTACCTCCAGAGCAGAAATGAAAAGTGGCGA470.15004948440443125No Hit
CGTAGTATCTATAGTACTGCTAGCGGGAGTTTTGTTCAGCATCCAGAACT470.15004948440443125No Hit
CGTAGTATCTATAGTACTGCTATTGGGACAGAAATTTGAGGAAATAAGGT470.15004948440443125No Hit
CGTAGTATCTATAGTACTGCTAGGGGGACGAAAACATAATGGACTCCAAC460.14685694218306036No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGAAAATGGACGAAGGACAAGGG450.1436643999616895No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCAGAAATTTGAGGAAATAAGGTG450.1436643999616895No Hit
CGTAGTATCTATAGTACTGCTACGGGGAAGAATATCAACAGGAACAGCAG440.14047185774031862No Hit
CGTAGTATCTATAGTACTGCTACCCCTCTTAGTTTGCATAGTTTCCCGTT440.14047185774031862No Hit
CGTAGTATCTATAGTACTGCTACCGCGAAGTAGGTACAGATGCAATTGTC430.13727931551894773No Hit
CGTAGTATCTATAGTACTGCTACGGGACAGAAATTTGAGGAAATAAGGTG420.13408677329757684No Hit
CGTAGTATCTATAGTACTGCTACGAGATCGGAAGAGCACACGTCTGAACT420.13408677329757684Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTAGTATCTATAGTACTGCTAGGGGGGAGAATATCAACAGGAACAGCAG420.13408677329757684No Hit
CGTAGTATCTATAGTACTGCTAGGGACAGAAATTTGAGGAAATAAGGTGG420.13408677329757684No Hit
CGTAGTATCTATAGTACTGCTATGCGGCACAGATTGAAAGCGACAGAGAA420.13408677329757684No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAATAGTTGGAGAAATTTCAC400.1277016888548351No Hit
CGTAGTATCTATAGTACTGCTAAGGCGAACTTCAGTGTAATCTTCAACCG400.1277016888548351No Hit
CGTAGTATCTATAGTACTGCTAGGGGCGGGGAGTCTTCGAGCTCTCGGAC400.1277016888548351No Hit
CGTAGTATCTATAGTACTGCTACCGAGGCCAAGGGTGTTGCCTCTTCCTT390.12450914663346424No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGGGAAAAC390.12450914663346424No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCAAAAACATAATGGACTCCAACA390.12450914663346424No Hit
CGTAGTATCTATAGTACTGCTAGCGAGGCCAAGGGTGTTGCCTCTTCCTT380.12131660441209335No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGAGTTTAA380.12131660441209335No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTATTT380.12131660441209335No Hit
CGTAGTATCTATAGTACTGCTAAGGGACAGAAATTTGAGGAAATAAGGTG370.11812406219072248No Hit
CGTAGTATCTATAGTACTGCTAGCGGGGGAAAACAAAAGCAACAAAAATG360.1149315199693516No Hit
CGTAGTATCTATAGTACTGCTAAGCGGGGGAAAACAAAAGCAACAAAAAT350.11173897774798072No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGAAAACAAAAGCAACAAAAATG350.11173897774798072No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAACAGTGACCACAGAAGCTG340.10854643552660984No Hit
CGTAGTATCTATAGTACTGCTACAGAGGCCAAGGGTGTTGCCTCTTCCTT340.10854643552660984No Hit
CGTAGTATCTATAGTACTGCTAGGGAGGCCAAGGGTGTTGCCTCTTCCTT340.10854643552660984No Hit
CGTAGTATCTATAGTACTGCTACAGGAGGGAAGAATATCAACAGGAACAG330.10535389330523896No Hit
CGTAGTATCTATAGTACTGCTAGCGAGGGCAATTGGGACAGAAATTTGAG320.10216135108386808No Hit
CGTAGTATCTATAGTACTGCTAGCGAGAGGAATTGGGACAGAAATTTGAG320.10216135108386808No Hit
CGTAGTATCTATAGTACTGCTAGGGGAGGGAAGAATATCAACAGGAACAG320.10216135108386808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA31050.094.840881
CTATAGT30950.094.536419
TAGTATC31050.094.38493
GTAGTAT31050.094.38492
AGTATCT31000.094.383924
GTATCTA30850.094.3809365
TCTATAG30850.094.3809368
ATCTATA30800.094.379947
TATCTAT30850.094.2269746
ATGGGTC205.34988E-447.49840554-55
CTACAGG404.116373E-947.49840520-21
TGGTCTA205.34988E-447.49840554-55
CTACACG301.4699526E-647.49840520-21
GGATGTG301.4699526E-647.49840542-43
TGTGAAC205.34988E-447.49840574-75
AGGGGAG600.047.49840522-23
GGGTCTC205.34988E-447.49840556-57
TAGTGTG205.34988E-447.49840560-61
TAGGAGG205.34988E-447.49840520-21
TCTAGAA301.4699526E-647.49840560-61
Produced by FastQC (version 0.11.5)