FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC307CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC307CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77368
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACAGAGTAGTACAGACCGGCGCTGGAGCTAGGATGAGTCCCAAT6350.8207527660014476No Hit
CGACATACAGAGTAGTACAGACCGGCACTGGAGCTAGGATGAGTCCCAAT4730.6113638713680074No Hit
CGACATACAGAGTAGTACAGACGCGTGATCAGTAGAAACAAGGGTGTTTT2420.31279081790921315No Hit
CGACATACAGAGTAGTACAGACAGGCCCCCTCAAAGCCGAGATCGCGCAG2220.2869403370902699No Hit
CGACATACAGAGTAGTACAGACCAGCGCTGGAGCTAGGATGAGTCCCAAT1950.2520421879846965No Hit
CGACATACAGAGTAGTACAGACGGGGGGGGAACAGTGACCACAGAAGCTG1830.2365318994933306No Hit
CGACATACAGAGTAGTACAGACCAGCACTGGAGCTAGGATGAGTCCCAAT1590.20551132251059873No Hit
CGACATACAGAGTAGTACAGACAGGCGCTGGAGCTAGGATGAGTCCCAAT1570.2029262744287044No Hit
CGACATACAGAGTAGTACAGACGGGGGGGGAACGCCAGGATGCCACAGAA1510.1951711301830214No Hit
CGACATACAGAGTAGTACAGACAGGCGAACAGAGGTTATAAGAATGATGG1170.1512253127908179No Hit
CGACATACAGAGTAGTACAGACCGGCCCTGGAGCTAGGATGAGTCCCAAT950.12278978388998035No Hit
CGACATACAGAGTAGTACAGACAGCAGAGGCCATGGAGGTTGCTAATCAG900.11632716368524454No Hit
CGACATACAGAGTAGTACAGACGCGTGATCAGTAGAAACAAGGTAGTTTT890.11503463964429739No Hit
CGACATACAGAGTAGTACAGACCCGCGCTGGAGCTAGGATGAGTCCCAAT870.11244959156240307No Hit
CGACATACAGAGTAGTACAGACGGGGGCAGAAATTTGAGGAAATAAGGTG870.11244959156240307No Hit
CGACATACAGAGTAGTACAGACCTGCGCTGGAGCTAGGATGAGTCCCAAT800.10340192327577293No Hit
CGACATACAGAGTAGTACAGACCGGCGCCAAACTCTCCTTATTTCTTCTT800.10340192327577293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGG201.735747E-594.975446
CATGCAG156.6011935E-494.975434
CATACGG156.6011935E-494.975434
CGACATA78000.092.228171
CAGAGTA78100.092.0568548
AGAGTAG78200.091.9998559
ACAGAGT78100.091.9960567
TACAGAG78650.091.714996
CATACAG78550.091.710844
ACATACA78750.091.417623
ATACAGA78950.091.3063355
GACATAC79550.090.498272
ATACAGG255.251005E-575.980355
ACGGAGT200.00206886971.231587
CGGAGTA200.00206886971.231588
ACAGGGT301.295256E-463.3169567
GCATACA551.166336E-860.4389153
TACGGAG250.005008758556.985266
TACATAG250.005008758556.985266
GGAGTAG250.005008758556.985269
Produced by FastQC (version 0.11.5)