FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC300CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC300CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67715
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTACACGCGTCGAGATCACGAGGCCAAGGGTGTTGCCTCTTCCTTTTA4520.6675035073469688No Hit
CGCTACACGCGTCGAGATCACGCGTGATCAGTAGAAACAAGGGTGTTTTT4450.6571660636491177No Hit
CGCTACACGCGTCGAGATCACGAGGGGGCCTGACGGGATGATAGAAAGAA2850.4208816362696596No Hit
CGCTACACGCGTCGAGATCACGGGGGCCTGACGGGATGATAGAAAGAACG2810.4149745255851731No Hit
CGCTACACGCGTCGAGATCACGCAAAGAGGGCCTATTATCTTTTGCCTAG1560.23037731669497155No Hit
CGCTACACGCGTCGAGATCACGCGGCCAAGGGTGTTGCCTCTTCCTTTTA1390.20527209628590418No Hit
CGCTACACGCGTCGAGATCACGGGGGGGGGAAAATGGACGAAGGACAAGG1370.20231854094366095No Hit
CGCTACACGCGTCGAGATCACGGAGGGGGGAAAATGGACGAAGGACAAGG1300.19198109724580964No Hit
CGCTACACGCGTCGAGATCACGGAAACCACTGACTGTCCAGCAGCAAAGT1240.18312043121907995No Hit
CGCTACACGCGTCGAGATCACGGGGCCAAGGGTGTTGCCTCTTCCTTTTA1180.17425976519235029No Hit
CGCTACACGCGTCGAGATCACGGGGGGAAGAATATCAACAGGAACAGCAG1130.1668758768367422No Hit
CGCTACACGCGTCGAGATCACGCAAAGCAGGGTGACAAAAACATAATGGA1070.15801521081001255No Hit
CGCTACACGCGTCGAGATCACGGAGGGGGCCTGACGGGATGATAGAAAGA1040.1535848777966477No Hit
CGCTACACGCGTCGAGATCACGAAAAGCAGGGTGACAAAAACATAATGGA1040.1535848777966477No Hit
CGCTACACGCGTCGAGATCACGGTGAGCGTGAACACAAATCCTAAAATTC950.1402938787565532No Hit
CGCTACACGCGTCGAGATCACGGGGGACAGAAATTTGAGGAAATAAGGTG940.13881710108543158No Hit
CGCTACACGCGTCGAGATCACGCCCCCACGCGTGATCAGTAGAAACAAGG900.13290999040094514No Hit
CGCTACACGCGTCGAGATCACGGGGGGGCCTGACGGGATGATAGAAAGAA870.1284796573875803No Hit
CGCTACACGCGTCGAGATCACGCAGAGACTGGAAAGTGTCTTTGCAGGAA860.12700287971645868No Hit
CGCTACACGCGTCGAGATCACGGTGGGGAGCGCCAGGATGCCACAGAAAT850.12552610204533707No Hit
CGCTACACGCGTCGAGATCACGCGTGATCAGTAGAAACAAGGTAGTTTTT850.12552610204533707No Hit
CGCTACACGCGTCGAGATCACGGGGGGCCTGACGGGATGATAGAAAGAAC790.11666543601860739No Hit
CGCTACACGCGTCGAGATCACGGGGGGCAGAAATTTGAGGAAATAAGGTG760.11223510300524256No Hit
CGCTACACGCGTCGAGATCACGATCAGCAAAAGCAGGGTGACAAAAACAT740.10928154766299933No Hit
CGCTACACGCGTCGAGATCACGGGGCACGGTGAGCGTGAACACAAATCCT740.10928154766299933No Hit
CGCTACACGCGTCGAGATCACGCGGGGGCCTGACGGGATGATAGAAAGAA700.10337443697851288No Hit
CGCTACACGCGTCGAGATCACGGGATGATAGAAAGAACGTACGTTTCGAC690.10189765930739127No Hit
CGCTACACGCGTCGAGATCACGGAGGAGGGAACAATAGTTGGAGAAATTT680.10042088163626965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTACA156.514488E-495.278531
GACGCGT156.591636E-494.997046
AGACGCG156.591636E-494.997045
GCGTCGG156.591636E-494.997049
CTAGACG156.591636E-494.997043
ATACACG156.591636E-494.997043
GCGTCGA66950.094.784219
CGCGTCG66950.094.784218
ACGCGTC67100.094.6431057
CGCTACA66850.094.637151
TACACGC67200.094.431594
CTACACG66950.094.429483
GCTACAC66950.094.429482
ACACGCG67100.094.3599555
CACGCGT69100.091.6288456
CGCTAGA255.1639574E-576.222821
GCTAGAC255.240352E-575.9976352
CGATACA200.002041756471.4588851
GATACAC200.002065884671.247792
CGCATCG200.002065884671.247798
Produced by FastQC (version 0.11.5)