FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC295CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC295CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14402
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACGATACACGCGTATGTACGGCGCCATCAAGGGAGCAATTTTACAA970.6735175670045828No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAGAATACTGACATCAGAG900.6249132064990973No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAGAATACTGACATCAGAGT890.6179697264268851No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAGAATACTGACATCAGAGT710.4929870851270657No Hit
CGATACGATACACGCGTATGTAGGAACAGGATACACCATGGACACAGTAA470.326343563393973No Hit
CGATACGATACACGCGTATGTAGCAAGGGGACGTAGTGTTGGTAATGAAA470.326343563393973No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAACAAAATAACATTCGAAG460.3194000833217609No Hit
CGATACGATACACGCGTATGTAGGGACAGAAATTTGAGGAAATAAGGTGG450.31245660324954866No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAAAACATAATGGACTCCAA430.2985696431051243No Hit
CGATACGATACACGCGTATGTAGGGGACAGAAATTTGAGGAAATAAGGTG420.29162616303291206No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAAACAAAAGCAACAAAAAT420.29162616303291206No Hit
CGATACGATACACGCGTATGTAGGGCAAAGAAGACAAGAGATAAGCATCA410.28468268296069993No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAATAGCAATAAGACCCAAA400.27773920288848775No Hit
CGATACGATACACGCGTATGTAGAGGAAGCAAAATTAAACAGAGAAGAAA340.23607832245521454No Hit
CGATACGATACACGCGTATGTAGGGCAAAGAAGACAAGAGATATGGCCCA310.215247882238578No Hit
CGATACGATACACGCGTATGTAGGGGGCCTGACGGGATGATAGAAAGAAC300.20830440216636578No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAGGAGGTGTCACTAAGCTA300.20830440216636578No Hit
CGATACGATACACGCGTATGTAGGGGGGCAAGAATACTGACATCAGAGTC290.2013609220941536No Hit
CGATACGATACACGCGTATGTACGGCGCAAAGAGGGCCTATTATCTTTTG290.2013609220941536No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAAAACATAATGGACTCCA280.19441744202194142No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAATAGTTGGAGAAATTTCAC280.19441744202194142No Hit
CGATACGATACACGCGTATGTAAACAGAACACACCAATACTCAGAAAAGA270.1874739619497292No Hit
CGATACGATACACGCGTATGTAGGGGAAGCAAAATTAAACAGAGAAGAAA260.180530481877517No Hit
CGATACGATACACGCGTATGTACGCCGCCATCAAGGGAGCAATTTTACAA250.1735870018053048No Hit
CGATACGATACACGCGTATGTAAGGCGCCATCAAGGGAGCAATTTTACAA240.16664352173309263No Hit
CGATACGATACACGCGTATGTAGGGAGAGCAATTGGGACAGAAATTTGAG230.15970004166088045No Hit
CGATACGATACACGCGTATGTAGGGGGAGCAATAGTTGGAGAAATTTCAC220.15275656158866824No Hit
CGATACGATACACGCGTATGTAGGGGGGCAAAAACATAATGGACTCCAAC220.15275656158866824No Hit
CGATACGATACACGCGTATGTACAGGAGGGAAGAATATCAACAGGAACAG210.14581308151645603No Hit
CGATACGATACACGCGTATGTAGGGGGCCAAGGAGGTGTCACTAAGCTAT210.14581308151645603No Hit
CGATACGATACACGCGTATGTAGGGGGGCCAAGGAGGTGTCACTAAGCTA210.14581308151645603No Hit
CGATACGATACACGCGTATGTACGGCCGGAAATAGCAATAAGACCCAAAG210.14581308151645603No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAATAGTTGGAGAAATTTCA210.14581308151645603No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAACAAAAGCAACAAAAATG200.13886960144424387No Hit
CGATACGATACACGCGTATGTACGGCAATCGCACGCAAAGAGGGCCTATT190.13192612137203166No Hit
CGATACGATACACGCGTATGTAGCGAGAGCAATTGGGACAGAAATTTGAG190.13192612137203166No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAATAGTTGGAGAAATTTCAC190.13192612137203166No Hit
CGATACGATACACGCGTATGTAGGGAAGGCAAGAGAAGAACCCCGCACTC190.13192612137203166No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAATACCACTATATGATGCAA190.13192612137203166No Hit
CGATACGATACACGCGTATGTAGGGGGGGAAAACAAAAGCAACAAAAATG180.12498264129981948No Hit
CGATACGATACACGCGTATGTAGGTACAGATGCAATTGTCGTTCTAAGTG170.11803916122760727No Hit
CGATACGATACACGCGTATGTAAGGGACAGAAATTTGAGGAAATAAGGTG170.11803916122760727No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAACAGTGACCACAGAAGCTG170.11803916122760727No Hit
CGATACGATACACGCGTATGTAAGGAAGCAAAATTAAACAGAGAAGAAAT170.11803916122760727No Hit
CGATACGATACACGCGTATGTAGGGGGACAAAAACATAATGGACTCCAAC170.11803916122760727No Hit
CGATACGATACACGCGTATGTACGGCCCCAAATAGGCCTCTAGATTGAAT170.11803916122760727No Hit
CGATACGATACACGCGTATGTACGGCCAGCAATGTTACATTTACCCAAAT160.11109568115539507No Hit
CGATACGATACACGCGTATGTAGGGGGGGAAATAGCAATAAGACCCAAAG160.11109568115539507No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAGAGAAGAACCCCGCACT160.11109568115539507No Hit
CGATACGATACACGCGTATGTAGGAAGCAAAATTAAACAGAGAAGAAATA160.11109568115539507No Hit
CGATACGATACACGCGTATGTAGGGGGGGAAACAAAATAACATTCGAAGC160.11109568115539507No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAATATATTCAATATGGAGA160.11109568115539507No Hit
CGATACGATACACGCGTATGTACAGAGCAGAAATGAAAAGTGGCGAGAGC160.11109568115539507No Hit
CGATACGATACACGCGTATGTAGGCAAGGGGACGTAGTGTTGGTAATGAA150.10415220108318289No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAAATAACATTCGAAGCAAC150.10415220108318289No Hit
CGATACGATACACGCGTATGTAGGGGGCGGAAATAGCAATAAGACCCAAA150.10415220108318289No Hit
CGATACGATACACGCGTATGTAGGGAAGCAAAATTAAACAGAGAAGAAAT150.10415220108318289No Hit
CGATACGATACACGCGTATGTAAGGAAGGCAAGAGAAGAACCCCGCACTC150.10415220108318289No Hit
CGATACGATACACGCGTATGTAGAGGGAAGAATATCAACAGGAACAGCAG150.10415220108318289No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAGGAGGTGTCACTAAGCT150.10415220108318289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG13900.094.984621
TACACGC14300.094.6643759
ATACACG14250.094.6632168
ATACGAT13950.094.656043
ACGATAC13950.094.656045
GATACGA13850.094.653592
TACGATA14000.094.317974
GATACAC14250.093.9965747
CGATACA14150.093.9894946
ATGGGAC205.3015794E-447.4982732-33
CAGTTGG205.3015794E-447.4982794-95
GCAAGCG205.3015794E-447.4982794-95
GTACCGC205.3015794E-447.4982720-21
GGCGCCA205.3015794E-447.4982724-25
GGCGCAC205.3015794E-447.4982724-25
TACGGGG403.983587E-947.4982720-21
CGCCATC205.3015794E-447.4982726-27
AGGCGCA205.3015794E-447.4982722-23
AAGGGGG205.3015794E-447.4982722-23
AGGGAGC205.3015794E-447.4982734-35
Produced by FastQC (version 0.11.5)